Radiosensitivity-Specific Proteomic and Signaling Pathway Network of Non-Small Cell Lung Cancer (NSCLC)
文献类型：期刊论文
| 作者 | Zhu, Xueru1; Wang, Yiting1; Jiang, Chang1; Li, Xiaoyang2; Sun, Linying3; Wang, Guangzhong3; Fu, Xiaolong1 |
| 刊名 | INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS
 |
| 出版日期 | 2022-02-01 |
| 卷号 | 112期号:2页码:529-541 |
| ISSN号 | 0360-3016 |
| DOI | 10.1016/j.ijrobp.2021.08.041 |
| 英文摘要 | Purpose: An unmet clinical need in non-small cell lung cancer (NSCLC) management is the accurate prediction of radiation response in patients receiving radical radiation therapy. We explored the intrinsic radiosensitivity of NSCLC from the proteomic profiles of NSCLC cell lines and paraffin-embedded human samples. Methods and Materials: To uncover radiosensitivity-specific proteomic and signaling pathways, we performed quantitative proteomics by data-independent acquisition mass spectrometry assay on 29 human NSCLC cell lines and 13 paraffin-embedded human NSCLC samples. We validated closely interacting radioresistant proteins by western blotting, immunofluorescence, real-time quantitative polymerase chain reaction in NSCLC cell lines, and immunohistochemistry in paraffinembedded human samples. We validated the functions of 3 key hub proteins by lentivirus transfection, clonogenic survival assay, and flow cytometry. Results: The proteomic profiling of NSCLC showed that the intrinsic radiosensitivity of NSCLC is mainly modulated by signaling pathways of proteoglycans in cancer, focal adhesion, and regulation of the actin cytoskeleton. We identified 71 differentially expressed proteins and validated 8 closely interacting proteins as radioresistant proteins of NSCLC. Moreover, we also validated the functionality of integrin-linked protein kinase, p21-activated kinase 1, and Ras GTPase-activating-like protein IQGAP1 in the radiation response of NSCLC cell lines. Finally, with the NSCLC radiosensitivity-specific proteins, we delineated the atlas network of NSCLC radiosensitivity-related signaling pathways. Conclusions: Radiosensitivity-specific proteins could guide individualized radiation therapy in clinical practice by predicting the radiation response of patients with NSCLC. Moreover, the NSCLC radiosensitivity-related signaling pathway atlas could guide further exploration of the underlying mechanism. (C) 2021 Elsevier Inc. All rights reserved. |
| 资助项目 | Shanghai Chest Hospital Project of Collaborative Innovation[YJXT20190101] ; National Natural Science Foundation of China[92059206] |
| WOS研究方向 | Oncology ; Radiology, Nuclear Medicine & Medical Imaging |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE INC |
| WOS记录号 | WOS:000740708800066 |
| 源URL | [http://119.78.100.204/handle/2XEOYT63/18277]  |
| 专题 | 中国科学院计算技术研究所期刊论文_英文 |
| 通讯作者 | Fu, Xiaolong |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Shanghai Chest Hosp, Dept Radiat Oncol, Shanghai, Peoples R China 2.Univ Sci & Technol China, Affiliated Hosp 1, Dept Radiat Oncol, Hefei, Anhui, Peoples R China 3.Chinese Acad Sci, Inst Comp Technol, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Xueru,Wang, Yiting,Jiang, Chang,et al. Radiosensitivity-Specific Proteomic and Signaling Pathway Network of Non-Small Cell Lung Cancer (NSCLC)[J]. INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS,2022,112(2):529-541. |
| APA | Zhu, Xueru.,Wang, Yiting.,Jiang, Chang.,Li, Xiaoyang.,Sun, Linying.,...&Fu, Xiaolong.(2022).Radiosensitivity-Specific Proteomic and Signaling Pathway Network of Non-Small Cell Lung Cancer (NSCLC).INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS,112(2),529-541. |
| MLA | Zhu, Xueru,et al."Radiosensitivity-Specific Proteomic and Signaling Pathway Network of Non-Small Cell Lung Cancer (NSCLC)".INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS 112.2(2022):529-541. |
入库方式： OAI收割
来源：计算技术研究所
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