Discovery of the correlation between the suspended membrane capacitance and adherent morphology of single cells enriching from clinical pleural effusion revealed by a microfluidic impedance flow cytometry
文献类型：期刊论文
| 作者 | Luan, Xiaofeng5,6; Li, Yuang5,6; Zhao, Haiping2; Sun, Sheng5,6; Fan, Yuanyuan5; Zhang, Wenchang5; Zhang, Lingqian5; Li, Mingxiao5; Wang, Jinghui3; Zhi, Tian4 |
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2022-11-15 |
| 卷号 | 371页码:11 |
| 关键词 | Pleural effusion (PE) 3D cell sieving Cell adherent morphology Elongation ratio Single -cell electrical properties Specific membrane capacitance |
| DOI | 10.1016/j.snb.2022.132487 |
| 英文摘要 | Cellular adherent morphology and suspended electrical property are two important intrinsic biophysical features of single cells in two states. However, few studies reported their relationship due to lacking systematic methods. Here, we proposed a toolchain for enriching, proliferating single cells from pleural effusions (PEs), and characterizing their adherent morphologies and suspended inherent electrical properties. Our 3D cell sieving device was employed to enrich rare tumor cells from every 50 mL clinical PEs. After proliferated, ten samples were enrolled, whose cells' adherent morphologies were quantified with the elongation ratio (Er). Our microfluidic impedance flow cytometry was developed to characterize similar to 65,400 suspended single cells' electrical properties (e.g., C-sm). Subsequently, we experimentally found that the C-sm of 5 spindle-like (mainly Er> 2) samples were all quantified as focused above 1.5 mu F/cm(2), whereas others' were all focused around 1-1.5 mu F/cm(2) for 5 round-like (mainly Er= 2) samples. Spearman rhos were introduced to further quantify this potential correlation from aspects of proportions (C-sm> 1.5 mu F/cm(2), Er> 2), average, and median, noting as 0.758 (p = 0.011), 0.760 (p = 0.011), and 0.744 (p = 0.014), respectively. Those results revealed a significant correlation between single cells' Er and C-sm-which means that the underlying correlation between cells' two label-free biophysical properties presented in two states was discovered. |
| 资助项目 | National Key Research and Devel-opment Program of China[2018YFC2001100] ; National Natural Science Foundation of China[62171441] ; Scientific Research and Equipment Development Project of CAS[YJKYYQ20210031] ; State Key Laboratory of Computer Architecture (ICT, CAS)[CARCH202122] ; Youth Innovation Promotion Association of ChineseAcademy of Sciences |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE SA |
| WOS记录号 | WOS:000843505600005 |
| 源URL | [http://119.78.100.204/handle/2XEOYT63/19464]  |
| 专题 | 中国科学院计算技术研究所期刊论文_英文 |
| 通讯作者 | Zhang, Lina; Zhao, Yang; Huang, Chengjun |
| 作者单位 | 1.Capital Med Univ, Beijing Chest Hosp, Beijing TB & Thorac Tumor Res Inst, Canc Res Ctr, Beijing 101125, Peoples R China 2.Xuanwu Hosp Capital Med Univ, Cerebrovascular Dis Res Inst, Beijing 100053, Peoples R China 3.Capital Med Univ, Beijing Chest Hosp, Beijing TB & Thorac Tumor Res Inst, Dept Med Oncol, Beijing 101125, Peoples R China 4.Chinese Acad Sci, Inst Comp Technol, State Key Lab Comp Architecture, Beijing 101125, Peoples R China 5.Chinese Acad Sci, Inst Microelect, Beijing 100029, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luan, Xiaofeng,Li, Yuang,Zhao, Haiping,et al. Discovery of the correlation between the suspended membrane capacitance and adherent morphology of single cells enriching from clinical pleural effusion revealed by a microfluidic impedance flow cytometry[J]. SENSORS AND ACTUATORS B-CHEMICAL,2022,371:11. |
| APA | Luan, Xiaofeng.,Li, Yuang.,Zhao, Haiping.,Sun, Sheng.,Fan, Yuanyuan.,...&Huang, Chengjun.(2022).Discovery of the correlation between the suspended membrane capacitance and adherent morphology of single cells enriching from clinical pleural effusion revealed by a microfluidic impedance flow cytometry.SENSORS AND ACTUATORS B-CHEMICAL,371,11. |
| MLA | Luan, Xiaofeng,et al."Discovery of the correlation between the suspended membrane capacitance and adherent morphology of single cells enriching from clinical pleural effusion revealed by a microfluidic impedance flow cytometry".SENSORS AND ACTUATORS B-CHEMICAL 371(2022):11. |
入库方式： OAI收割
来源：计算技术研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。