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Recent Progress in Chemical Cross-linking Coupled With Mass Spectrometry

文献类型：期刊论文

作者Chen Zhen-Lin1,2; Cao Yong3; He Si-Min1,2
刊名PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
出版日期2022-04-01
卷号49期号:4页码:737-758
关键词cross-linking mass spectrometry protein structure protein interaction proteomics mass spectrometry
ISSN号1000-3282
DOI10.16476/j.pibb.2021.0057
英文摘要Chemical cross-linking coupled with mass spectrometry (CXMS) is an important tool to analyze protein structures and protein-protein interactions. In the last five years, CXMS has made great progress in both methods and applications. In terms of methods, on the one hand, cleavable cross-linkers and new separation and enrichment methods have shown good prospects, and on the other hand, more efficient cross-linked peptide search engines and quality control methods provide powerful tools for CXMS data analysis. In terms of applications, on the one hand, CXMS combined with cryo-electron microscopy has determined a large number of protein structures, and on the other hand, CXMS has shown the potential to analyze protein-protein interaction networks at a proteome scale. The intensive research on CXMS in methods and applications reflect the important role of this technology. Here we review the various aspects of CXMS, including cross-linker selection, cross-linking reaction, protein digestion, separation and enrichment, data acquisition, cross-linked peptide identification, quality control, and application, and mainly focus on progress in the last five years. Lastly, we discuss the challenges and opportunities of CXMS in the future. In section 1, we review cross-linkers from the aspects of reactive group and spacer arm. In section 2, we give tips for the cross-linking reaction. In section 3, we describe the sequential digestion strategy for cross-linked proteins. In section 4, we elaborate enrichment methods for cross-linked peptides, including affinity purification, chromatographic separation, and ion mobility mass spectrometry. In section 5, we elaborate data acquisition methods for cross-linked peptides, and compare three methods for MS-cleavable cross-linked peptides. In section 6, we elaborate search engines for cross-linked peptide identification. In section 7, we describe quality control methods for cross-linked peptide identification. In section 8, we review applications of CXMS and list some proteome-wide CXMS studies. In section 9, we conclude the paper and discuss the challenges and opportunities of CXMS in the future.
资助项目National Natural Science Foundation of China[32071435] ; National Basic Research Program of China[2016YFA0501300]
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
语种英语
WOS记录号WOS:000794195500011
出版者CHINESE ACAD SCIENCES, INST BIOPHYSICS
源URL[http://119.78.100.204/handle/2XEOYT63/19556]  
专题中国科学院计算技术研究所期刊论文_英文
通讯作者He Si-Min
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Comp Technol, Key Lab Intelligent Informat Proc, Beijing 100190, Peoples R China
3.Natl Inst Biol Sci, Beijing 102206, Peoples R China
推荐引用方式
GB/T 7714		 Chen Zhen-Lin,Cao Yong,He Si-Min. Recent Progress in Chemical Cross-linking Coupled With Mass Spectrometry[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2022,49(4):737-758.
APA Chen Zhen-Lin,Cao Yong,&He Si-Min.(2022).Recent Progress in Chemical Cross-linking Coupled With Mass Spectrometry.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,49(4),737-758.
MLA Chen Zhen-Lin,et al."Recent Progress in Chemical Cross-linking Coupled With Mass Spectrometry".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 49.4(2022):737-758.

入库方式： OAI收割

来源：计算技术研究所

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