A self-assembled polydopamine film on the surface of magnetic nanoparticles for specific capture of protein
文献类型:期刊论文
作者 | Zhang, Min1,2; Zhang, Xihao1,2; He, Xiwen1,2; Chen, Langxing1,2; Zhang, Yukui1,2,3 |
刊名 | nanoscale
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出版日期 | 2012 |
卷号 | 4期号:10页码:3141-3147 |
产权排序 | 3,5 |
通讯作者 | 陈朗星 |
英文摘要 | in this study, we report a facile method for the preparation of core-shell magnetic molecularly imprinted polymers (mips) for protein recognition. uniform carboxyl group functionalized fe3o4 nanoparticles (nps) were synthesized using a solvothermal method. magnetic mips were synthesized by self-polymerization of dopamine in the presence of template protein on the surface of the fe3o4 nps. a thin layer of polydopamine can be coated on fe3o4 nps via dopamine self-polymerization and the imprinted polydopamine shells can be controlled by the mass ratio of fe3o4 nps and dopamine. more importantly, there is a critical value of polydopamine shell thickness for the maximum rebinding capacity. the as-prepared lysozyme-imprinted fe3o4@polydopamine nps show high binding capacity and acceptable specific recognition behavior towards template proteins. this method provides the possibility for the separation and enrichment of abundant proteins in proteomic analysis. |
WOS标题词 | science & technology ; physical sciences ; technology |
学科主题 | 物理化学 |
类目[WOS] | chemistry, multidisciplinary ; nanoscience & nanotechnology ; materials science, multidisciplinary ; physics, applied |
研究领域[WOS] | chemistry ; science & technology - other topics ; materials science ; physics |
关键词[WOS] | molecularly imprinted polymers ; histidine-tagged proteins ; iron-oxide nanoparticles ; bovine hemoglobin ; biomedical applications ; sorbent assays ; aqueous-media ; human serum ; recognition ; shell |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000303604000024 |
公开日期 | 2013-10-11 |
源URL | [http://159.226.238.44/handle/321008/118171] ![]() |
专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
作者单位 | 1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China 2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Min,Zhang, Xihao,He, Xiwen,et al. A self-assembled polydopamine film on the surface of magnetic nanoparticles for specific capture of protein[J]. nanoscale,2012,4(10):3141-3147. |
APA | Zhang, Min,Zhang, Xihao,He, Xiwen,Chen, Langxing,&Zhang, Yukui.(2012).A self-assembled polydopamine film on the surface of magnetic nanoparticles for specific capture of protein.nanoscale,4(10),3141-3147. |
MLA | Zhang, Min,et al."A self-assembled polydopamine film on the surface of magnetic nanoparticles for specific capture of protein".nanoscale 4.10(2012):3141-3147. |
入库方式: OAI收割
来源:大连化学物理研究所
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