A compact and highly sensitive light-emitting diode-induced fluorescence detector for capillary flow systems
文献类型：期刊论文
| 作者 | Geng, Xuhui1,2; Wu, Dapeng1; Guan, Yafeng1 |
| 刊名 | talanta
 |
| 出版日期 | 2012-01-15 |
| 卷号 | 88期号:15页码:463-467 |
| 关键词 | Light-emitting diode Fluorescence detector Compact |
| 产权排序 | 1,1 |
| 通讯作者 | 关亚风 |
| 英文摘要 | a compact and highly sensitive light-emitting diode-induced fluorescence detector (led-fd) was constructed and evaluated. the led excitation beam was restricted by a 200 mu m diameter pinhole on a 55 mu m thick lighttight film. the led, excitation filter, pinhole, and capillary flow cell were placed tightly against each other without either optical lens or excitation fiber. the performance of the led-fd was evaluated by flow injection analysis (fia) of sodium fluorescein solution. the limit of detection (lod) was determined as 0.75 nm (snr= 3). compared with our previous work, a 3.5-fold enhancement on the signal-to-noise ratio (snr) was obtained. the system exhibited linear responses in the concentration range of 2-200 nm (r = 0.9993). the error of repeatability was within 2.0% r.s.d. on peak height in a ten-repeated injection of 5 nm solution. the reproducibility between devices was within 2.2% r.s.d. on peak height of 10 nm fluorescein solution. finally, the led-fd was compared with commercial fluorescence detector, and similar fluorescence signal intensity was obtained. (c) 2011 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | optical-fiber ; electrophoresis ; performance |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301159400064 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/118224]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Instrumentat & Analyt Chem, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Geng, Xuhui,Wu, Dapeng,Guan, Yafeng. A compact and highly sensitive light-emitting diode-induced fluorescence detector for capillary flow systems[J]. talanta,2012,88(15):463-467. |
| APA | Geng, Xuhui,Wu, Dapeng,&Guan, Yafeng.(2012).A compact and highly sensitive light-emitting diode-induced fluorescence detector for capillary flow systems.talanta,88(15),463-467. |
| MLA | Geng, Xuhui,et al."A compact and highly sensitive light-emitting diode-induced fluorescence detector for capillary flow systems".talanta 88.15(2012):463-467. |
入库方式： OAI收割
来源：大连化学物理研究所
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