High-level secretory expression and characterization of the recombinant Kluyveromyces marxianus inulinase
文献类型：期刊论文
| 作者 | Zhang, Sufang1,2; Yang, Fan1; Wang, Qian1,2; Hua, Yanyan1; Zhao, Zongbao K.1,2 |
| 刊名 | process biochemistry
 |
| 出版日期 | 2012 |
| 卷号 | 47期号:1页码:151-155 |
| 关键词 | Inulinase Secretory expression Inulin Pichia pastoris Enzyme activity |
| 产权排序 | 1,1 |
| 通讯作者 | 赵宗保 |
| 英文摘要 | inulinase is an important enzyme used in the high fructose syrup and other related industries. a more cost-effective approach is required for producing highly active inulinase. in this study, the gene encoding inulinase of the yeast kluyveromyces marxianus cbs 6556 was expressed in methylotrophic host pichia pastoris and secretory production of recombinant inulinase (rkminu) in the yeast under methanol induction was achieved. the purified rkminu showed a specific activity of 2714 u/mg, which is over 12-fold higher than those of other inulinases described previously. it displayed excellent stability from 30 to 50 degrees c and ph 3.0-5.0, and the half-life of rkminu was over 96 under these conditions. moreover, rkminu saccharified jerusalem artichoke tuber juice effectively. (c) elsevier ltd. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; technology |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology ; engineering, chemical |
| 研究领域[WOS] | biochemistry & molecular biology ; biotechnology & applied microbiology ; engineering |
| 关键词[WOS] | yeast pichia-pastoris ; aspergillus-niger ; extracellular inulinase ; gene ; purification ; cloning ; proteins ; cbs-6556 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000300133000022 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/118274]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.CAS, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Sufang,Yang, Fan,Wang, Qian,et al. High-level secretory expression and characterization of the recombinant Kluyveromyces marxianus inulinase[J]. process biochemistry,2012,47(1):151-155. |
| APA | Zhang, Sufang,Yang, Fan,Wang, Qian,Hua, Yanyan,&Zhao, Zongbao K..(2012).High-level secretory expression and characterization of the recombinant Kluyveromyces marxianus inulinase.process biochemistry,47(1),151-155. |
| MLA | Zhang, Sufang,et al."High-level secretory expression and characterization of the recombinant Kluyveromyces marxianus inulinase".process biochemistry 47.1(2012):151-155. |
入库方式： OAI收割
来源：大连化学物理研究所
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