Chip-based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids
文献类型：期刊论文
| 作者 | Chang, Yuwei; Zhao, Chunxia; Wu, Zeming; Zhou, Jia; Zhao, Sumin; Lu, Xin; Xu, Guowang |
| 刊名 | electrophoresis
 |
| 出版日期 | 2012-08-01 |
| 卷号 | 33期号:15页码:2399-2406 |
| 关键词 | Flavonoids HPLC-chip MS Metabolic profiling Soybean |
| 产权排序 | 1, 1 |
| 通讯作者 | 赵春霞 |
| 英文摘要 | in this work a chip-based nano hplc coupled ms (hplc-chip/ms) method with a simple sample preparation procedure was developed for the flavonoid profiling of soybean. the analytical properties of the method including the linearity (r2, 0.9920.995), reproducibility (rsd, 1.507.66%), intraday precision (rsd, 1.415.14%) and interday precision (rsd, 2.7616.90%) were satisfactory. compared with the conventional hplc/ms method, a fast extraction and analysis procedure was applied and more flavonoids were detected in a single run. additionally, 13 flavonoids in soybean seed were identified for the first time. the method was then applied to the profiling of six varieties of soybean sowed at the same place. a clear discrimination was observed among different cultivars, three isoflavones, accounting for nearly 80% of total flavonoid contents, were found increased in the spring soybeans compared with the summer cultivars. |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | integrated microfluidic device ; antioxidant activity ; isoflavone content ; phenolic-compounds ; ion-source ; quercetin ; identification ; kaempferol ; seeds ; naringenin |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000307399400018 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/118316]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chang, Yuwei,Zhao, Chunxia,Wu, Zeming,et al. Chip-based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids[J]. electrophoresis,2012,33(15):2399-2406. |
| APA | Chang, Yuwei.,Zhao, Chunxia.,Wu, Zeming.,Zhou, Jia.,Zhao, Sumin.,...&Xu, Guowang.(2012).Chip-based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids.electrophoresis,33(15),2399-2406. |
| MLA | Chang, Yuwei,et al."Chip-based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids".electrophoresis 33.15(2012):2399-2406. |
入库方式： OAI收割
来源：大连化学物理研究所
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