Improved Catalytic Performance of Lipase Accommodated in the Mesoporous Silicas with Polymer-Modified Microenvironment
文献类型：期刊论文
| 作者 | Liu, Jia1,2; Bai, Shiyang1,2; Jin, Qianru1,2; Zhong, Hua1; Li, Can1; Yang, Qihua1 |
| 刊名 | langmuir
 |
| 出版日期 | 2012-06-26 |
| 卷号 | 28期号:25页码:9788-9796 |
| 产权排序 | 1,1 |
| 通讯作者 | 杨启华 ; 李灿 |
| 英文摘要 | the highly ordered mesoporous silicas with elaborately controlled microenvironment were synthesized via covalent incorporation of long-chain polymers (m-w = 2000 g mol(-1)) bearing specific hydrophilic/hydrophobic balance. the microenvironment (hydrophilicity/hydrophobicity) of the mesoporous silicas was quantitatively determined by gas adsorption experiments and investigated by lysozyme (lyz) adsorption. the relative activity of lipase from pseudomonas cepacia (pcl) encapsulated in the mesoporous silica with moderate hydrophobic microenvironment (hereafter denoted as mhm) reaches up to 281% compared with the free pcl, notably higher than that of pcl accommodated in the mesoporous silicas with hydrophilic or strong hydrophobic microenvironment (20.7-26.2% relative to the free pcl). moreover, pcl entrapped in the nanochannels with mhm affords the highest initial rate in the kinetic resolution of (r,s)-1-phenylethanol relative to other immobilized pcl. the above results suggest that the mhm could render the active center of pcl entirely exposed to the substrates without interrupting its native conformation in the "interfacial activation". in addition, the nano channels with mhm could markedly improve the thermal stability of pcl (preserving nearly 60% of the initial activity after the incubation at 70 degrees c for 2 h) and facilitate the recycling of the immobilized pcl in both aqueous and organic media. our work demonstrates that the subtle modulation of the microenvironment of mesoporous silicas for enzyme immobilization designates a very promising strategy to fabricate the highly active and stable heterogeneous biocatalysts for industrial application. |
| WOS标题词 | science & technology ; physical sciences ; technology |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, multidisciplinary ; chemistry, physical ; materials science, multidisciplinary |
| 研究领域[WOS] | chemistry ; materials science |
| 关键词[WOS] | rhizomucor-miehei lipase ; sol-gel materials ; lysozyme adsorption ; interfacial adsorption ; hydrophobic supports ; immobilization ; enzymes ; hyperactivation ; biocatalysis |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000305661400073 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/118384]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Catalysis, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Jia,Bai, Shiyang,Jin, Qianru,et al. Improved Catalytic Performance of Lipase Accommodated in the Mesoporous Silicas with Polymer-Modified Microenvironment[J]. langmuir,2012,28(25):9788-9796. |
| APA | Liu, Jia,Bai, Shiyang,Jin, Qianru,Zhong, Hua,Li, Can,&Yang, Qihua.(2012).Improved Catalytic Performance of Lipase Accommodated in the Mesoporous Silicas with Polymer-Modified Microenvironment.langmuir,28(25),9788-9796. |
| MLA | Liu, Jia,et al."Improved Catalytic Performance of Lipase Accommodated in the Mesoporous Silicas with Polymer-Modified Microenvironment".langmuir 28.25(2012):9788-9796. |
入库方式： OAI收割
来源：大连化学物理研究所
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