SUV39H1 orchestrates temporal dynamics of centromeric methylation essential for faithful chromosome segregation in mitosis
文献类型：期刊论文
| 作者 | Chu, Lingluo3; Zhu, Tongge1,3; Liu, Xing3,5; Yu, Ruoying3; Bacanamwo, Methode5; Dou, Zhen1,3; Chu, Youjun1,3; Zou, Hanfa4; Gibbons, Gary H.5; Wang, Dongmei3 |
| 刊名 | journal of molecular cell biology
 |
| 出版日期 | 2012-10-01 |
| 卷号 | 4期号:5页码:331-340 |
| 关键词 | mitosis SUV39H1 methylation MARC syntelin |
| 通讯作者 | xiading ; xuebiaoyao |
| 产权排序 | 3,8 |
| 英文摘要 | histone methylation performs multiple functions such as dna replication, transcription regulation, heterochromatin formation, and chromatin condensation. how this methylation gradient is orchestrated in the centromere during chromosome segregation is not known. here we examine the temporal dynamics of protein methylation in the centromere by suv39h1 methyltransferase, a key mitotic regulator, using fluorescence resonance energy transfer-based sensors in living hela cells and immunofluorescence of native suv39h1 substrates. a quantitative analysis of methylation dynamics, using centromere-targeted sensors, reveals a temporal change during chromosome segregation. these dynamics result in an accurate chromosome congression to and alignment at the equator as an inhibition of methylation dynamics using suv39h1 inhibitor perturbs chromosome congression in living hela cells. surprisingly, this inhibition of methylation results in a brief increase in aurora b kinase activity and an enrichment of microtubule depolymerase mcak in the centromere with a concomitant kinetochoremicrotubule destabilization and a reduced tension across the sister kinetochores with ultimate chromosome misalignments. we reason that suv39h1 generates a gradient of methylation marks at the kinetochore that provides spatiotemporal information essential for accurate chromosome segregation in mitosis. |
| 学科主题 | 物理化学 |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | cell biology |
| 研究领域[WOS] | cell biology |
| 关键词[WOS] | aurora-b kinase ; cenp-e ; mitotic checkpoint ; spindle microtubules ; cell-division ; protein e ; phosphorylation ; kinetochore ; attachment ; cancer |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000309707000007 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/118427]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Beijing Univ Chinese Med, Beijing 100027, Peoples R China 2.Univ Sci & Technol China, Anhui Key Lab Cellular Dynam & Chem Biol, Sch Life Sci, Anhua 230026, Peoples R China 3.Univ Sci & Technol China, Anhui Key Lab Cellular Dynam & Chem Biol, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 4.Dalian Inst Phys Chem, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 5.Morehouse Sch Med, Dept Physiol, Atlanta, GA 30310 USA |
| 推荐引用方式 GB/T 7714 | Chu, Lingluo,Zhu, Tongge,Liu, Xing,et al. SUV39H1 orchestrates temporal dynamics of centromeric methylation essential for faithful chromosome segregation in mitosis[J]. journal of molecular cell biology,2012,4(5):331-340. |
| APA | Chu, Lingluo.,Zhu, Tongge.,Liu, Xing.,Yu, Ruoying.,Bacanamwo, Methode.,...&Yao, Xuebiao.(2012).SUV39H1 orchestrates temporal dynamics of centromeric methylation essential for faithful chromosome segregation in mitosis.journal of molecular cell biology,4(5),331-340. |
| MLA | Chu, Lingluo,et al."SUV39H1 orchestrates temporal dynamics of centromeric methylation essential for faithful chromosome segregation in mitosis".journal of molecular cell biology 4.5(2012):331-340. |
入库方式： OAI收割
来源：大连化学物理研究所
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