Specific and Sensitive Fluorescence Anisotropy Sensing of Guanine-Quadruplex Structures via a Photoinduced Electron Transfer Mechanism
文献类型：期刊论文
| 作者 | Zhang, Dapeng1; Shen, Hujun2; Li, Guohui2; Zhao, Bailin1; Yu, Anchi4; Zhao, Qiang3; Wang, Hailin1 |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2012-09-18 |
| 卷号 | 84期号:18页码:8088-8094 |
| ISSN号 | 0003-2700 |
| 产权排序 | 2,2 |
| 通讯作者 | wanghailin |
| 英文摘要 | fluorescence anisotropy (fa) is a homogeneous, ratiometric, and real-time analytical technology. by selective labeling of a guanine (g)-quadruplex motif with tetramethylrhodamine (tmr), here, it is established that a large reduction in fa response can be specifically associated with the unfolding -> folding transition of g-quadruplex structures. on the basis of fluorescence intensity, polarization and lifetime analysis, and molecular docking simulation, the mechanism was found to be that the labeled fluorophore (tmr) can intramolecularly interact with adjacent g bases in an unfolded g-quadruplex motif, which allows for the photoinduced electron transfer (pet) occurring between the fluorophore and g bases, leading to a short fluorescence lifetime. upon the folding of the motif to form a stable g-quadruplex structure, the intramolecular interactions and the concomitant pet could be eliminated with an increased fluorescence lifetime, leading to a large reduction in the fa response. on the basis of this mechanism, a novel, specific, and sensitive fa approach was developed for the detection of biologically and functionally important g-quadruplex structures. the approach is examined and validated using one normal g-quadruplex motif, five mutants, and six small cations and is potentially applicable to the study of g-quadruplexes at single molecule level, ligand screening, profiling of highly ordered dna nanostructures, and biosensing. |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | circular-dichroism ; potassium-ion ; dna aptamer ; molecular-biology ; human thrombin ; polarization ; stability ; base ; oligodeoxyribonucleotides ; nanoparticles |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000308829700073 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/118448]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Environm Chem & Ecotoxicol, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian 116023, Peoples R China 3.Shanxi Univ, Res Ctr Environm Sci & Engn, Taiyuan 030006, Shanxi Province, Peoples R China 4.Renmin Univ China, Dept Chem, Beijing 100872, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Dapeng,Shen, Hujun,Li, Guohui,et al. Specific and Sensitive Fluorescence Anisotropy Sensing of Guanine-Quadruplex Structures via a Photoinduced Electron Transfer Mechanism[J]. analytical chemistry,2012,84(18):8088-8094. |
| APA | Zhang, Dapeng.,Shen, Hujun.,Li, Guohui.,Zhao, Bailin.,Yu, Anchi.,...&Wang, Hailin.(2012).Specific and Sensitive Fluorescence Anisotropy Sensing of Guanine-Quadruplex Structures via a Photoinduced Electron Transfer Mechanism.analytical chemistry,84(18),8088-8094. |
| MLA | Zhang, Dapeng,et al."Specific and Sensitive Fluorescence Anisotropy Sensing of Guanine-Quadruplex Structures via a Photoinduced Electron Transfer Mechanism".analytical chemistry 84.18(2012):8088-8094. |
入库方式： OAI收割
来源：大连化学物理研究所
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