Establishment of reliable mass spectra and retention indices library: Identification of fatty acids in human plasma without authentic standards
文献类型：期刊论文
| 作者 | Zhang, Liangxiao1,2; Tan, Binbin3; Zeng, Maomao4; Lu, Hongmei1; Liang, Yizeng1 |
| 刊名 | talanta
 |
| 出版日期 | 2012-01-15 |
| 卷号 | 88页码:311-317 |
| 关键词 | Reliable mass spectra and retention indices library Identification Fatty acids Human plasma GC-MS |
| 产权排序 | 2,1 |
| 通讯作者 | yizengliang |
| 英文摘要 | gas chromatography mass spectrometry (gc-ms) is routinely employed to analyze small molecules in various samples. the more challenge of gc-ms data processing is to identify the unknown compounds in samples. mass spectra and retention indices library searching are commonly used method. however, the current libraries are often built through collecting data from different groups. to unknown compounds with similar mass spectra and retention indices (e.g. geometric (cis/trans) isomers), the inaccurate results sometime are supplied. in this case, the costly standard compounds have to be used in every analysis. in this report, taking identification of fatty acids as an example, we proposed a strategy of establishment of special database constructed by equivalent chain length (ecl) values in uniform conditions and mass spectra of fatty acid methyl esters (fames). the mass spectral characteristics were firstly used to identify all expected straight saturated fatty acids, and subsequently calculate the ecl for fatty acids in the sample. finally, the ecl values of fatty acids in the sample were compared with those of fatty acids in the customized database to identify their structures. the results showed that the method developed in this report could effectively identify similar unknown compounds (fames in the human plasma) after validated by the authentic standards. (c) 2011 elsevier b.v. all rights reserved. |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | evolving latent projections ; 2-way multicomponent data ; gas-chromatography ; adipose-tissue ; methyl-esters ; spectrometry ; derivatives ; resolution ; obesity |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301159400041 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/118452]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 3.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Key Lab Syst Biomed, Minist Educ, Shanghai 200240, Peoples R China 4.Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Liangxiao,Tan, Binbin,Zeng, Maomao,et al. Establishment of reliable mass spectra and retention indices library: Identification of fatty acids in human plasma without authentic standards[J]. talanta,2012,88:311-317. |
| APA | Zhang, Liangxiao,Tan, Binbin,Zeng, Maomao,Lu, Hongmei,&Liang, Yizeng.(2012).Establishment of reliable mass spectra and retention indices library: Identification of fatty acids in human plasma without authentic standards.talanta,88,311-317. |
| MLA | Zhang, Liangxiao,et al."Establishment of reliable mass spectra and retention indices library: Identification of fatty acids in human plasma without authentic standards".talanta 88(2012):311-317. |
入库方式： OAI收割
来源：大连化学物理研究所
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