Enhanced top-down characterization of histone post-translational modifications
文献类型：期刊论文
| 作者 | Tian, Zhixin1,2; Tolic, Nikola1; Zhao, Rui1; Moore, Ronald J.3; Hengel, Shawna M.1; Robinson, Errol W.1; Stenoien, David L.3; Wu, Si1; Smith, Richard D.3; Pasa-Tolic, Ljiljana1 |
| 刊名 | genome biology
 |
| 出版日期 | 2012 |
| 卷号 | 13期号:10页码:1 |
| 关键词 | Saltless WCX-HILIC top-down histone posttranslational modification |
| 产权排序 | 2,1 |
| 通讯作者 | ljiljanapaš ; atolić |
| 英文摘要 | post-translational modifications (ptms) of core histones work synergistically to fine tune chromatin structure and function, generating a so-called histone code that can be interpreted by a variety of chromatin interacting proteins. we report a novel online two-dimensional liquid chromatography-tandem mass spectrometry (2d lc-ms/ms) platform for high-throughput and sensitive characterization of histone ptms at the intact protein level. the platform enables unambiguous identification of 708 histone isoforms from a single 2d lc-ms/ms analysis of 7.5 mu g purified core histones. the throughput and sensitivity of comprehensive histone modification characterization is dramatically improved compared with more traditional platforms. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | biotechnology & applied microbiology ; genetics & heredity |
| 研究领域[WOS] | biotechnology & applied microbiology ; genetics & heredity |
| 关键词[WOS] | mass-spectrometry ; liquid-chromatography ; h4 ; isoforms ; repair ; domain ; code ; h3 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000313183900005 |
| 公开日期 | 2013-10-11 |
| 源URL | [http://159.226.238.44/handle/321008/118454]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Pacific NW Natl Lab, Environm Mol Sci Lab, Richland, WA 99352 USA 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China 3.Pacific NW Natl Lab, Biol Sci Div, Richland, WA 99352 USA |
| 推荐引用方式 GB/T 7714 | Tian, Zhixin,Tolic, Nikola,Zhao, Rui,et al. Enhanced top-down characterization of histone post-translational modifications[J]. genome biology,2012,13(10):1. |
| APA | Tian, Zhixin.,Tolic, Nikola.,Zhao, Rui.,Moore, Ronald J..,Hengel, Shawna M..,...&Pasa-Tolic, Ljiljana.(2012).Enhanced top-down characterization of histone post-translational modifications.genome biology,13(10),1. |
| MLA | Tian, Zhixin,et al."Enhanced top-down characterization of histone post-translational modifications".genome biology 13.10(2012):1. |
入库方式： OAI收割
来源：大连化学物理研究所
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