IDENTIFICATION OF A GENOMIC REGION LINKED WITH SEX DETERMINATION OF UNDARIA PINNATIFIDA (ALARIACEAE) THROUGH GENOMIC RESEQUENCING AND GENETIC LINKAGE ANALYSES OF A SEGREGATING GAMETOPHYTE FAMILY
文献类型：期刊论文
| 作者 | Shan, Tifeng1,2; Li, Yuqian1,3; Pang, Shaojun1,2 |
| 刊名 | JOURNAL OF PHYCOLOGY
 |
| 出版日期 | 2022-11-29 |
| 页码 | 11 |
| ISSN号 | 0022-3646 |
| 关键词 | brown alga genetic linkage map kelp monoecious sex determination SNP |
| DOI | 10.1111/jpy.13295 |
| 通讯作者 | Pang, Shaojun(sjpang@qdio.ac.cn) |
| 英文摘要 | Different from the traditional knowledge about kelp, three sexual phenotypes (female, male, and monoecious) exist in the haploid gametophytes of Undaria pinnatifida. However, the sex-determining mechanisms remain unknown. Genetic linkage mapping is an efficient tool to identify sex-linked regions. In the present study, we resequenced a segregating gametophyte family based on the male genome of U. pinnatifida. A high-density genetic linkage map was constructed using 9887 SNPs, with an average distance of 0.41 cM between adjacent SNPs. On the basis of this genetic map and using the composite interval mapping method, we identified 62 SNPs significantly linked with the sexual phenotype. They were located at a position of 67.67 cM on the linkage group 23, corresponding to a physical range of 14.67 Mbp on the HiC_Scaffold_23 of the genome. Reanalysis of the previous specific length amplified fragment sequencing data according to the reference genome led to the identification of a sex-linked genomic region that encompassed the above-mentioned 14.67 Mbp region. Hence, this overlapped genomic range was likely the sex-determining region. Within this region, 129 genes were retrieved and 39 of them were annotated with explicit function, including the potential male sex-determining gene-encoding high mobility group (HMG) domain protein. Relative expression analysis of the HMG gene showed that its expression was higher in male gametophytes during the vegetative phase and monoecious gametophytes during both the vegetative and gametogenesis phases, but significantly lower in male gametophytes during the gametogenesis phase. These results provide a foundation for deciphering the sex-determining mechanism of U. pinnatifida. |
| 资助项目 | National Natural Science Foundation of China[42176100] ; Chinese Academy of Sciences[KFJ-BRP-017-27] ; China Agriculture Research System[CARS-50] ; Taishan Scholar Program of Shandong Province ; Foundation for Huiquan Scholar of Institute of Oceanology, Chinese Academy of Sciences |
| WOS研究方向 | Plant Sciences ; Marine & Freshwater Biology |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000892266300001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/180237]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Pang, Shaojun |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shan, Tifeng,Li, Yuqian,Pang, Shaojun. IDENTIFICATION OF A GENOMIC REGION LINKED WITH SEX DETERMINATION OF UNDARIA PINNATIFIDA (ALARIACEAE) THROUGH GENOMIC RESEQUENCING AND GENETIC LINKAGE ANALYSES OF A SEGREGATING GAMETOPHYTE FAMILY[J]. JOURNAL OF PHYCOLOGY,2022:11. |
| APA | Shan, Tifeng,Li, Yuqian,&Pang, Shaojun.(2022).IDENTIFICATION OF A GENOMIC REGION LINKED WITH SEX DETERMINATION OF UNDARIA PINNATIFIDA (ALARIACEAE) THROUGH GENOMIC RESEQUENCING AND GENETIC LINKAGE ANALYSES OF A SEGREGATING GAMETOPHYTE FAMILY.JOURNAL OF PHYCOLOGY,11. |
| MLA | Shan, Tifeng,et al."IDENTIFICATION OF A GENOMIC REGION LINKED WITH SEX DETERMINATION OF UNDARIA PINNATIFIDA (ALARIACEAE) THROUGH GENOMIC RESEQUENCING AND GENETIC LINKAGE ANALYSES OF A SEGREGATING GAMETOPHYTE FAMILY".JOURNAL OF PHYCOLOGY (2022):11. |
入库方式： OAI收割
来源：海洋研究所
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