A Non-Canonical Teleost NK-Lysin: Antimicrobial Activity via Multiple Mechanisms
文献类型：期刊论文
| 作者 | Xu, Hang1,2,3,4 ; Yuan, Zihao1,2,3; Sun, Li1,2,3,4
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| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2022-11-01 |
| 卷号 | 23期号:21页码:15 |
| 关键词 | NK-lysin Paralichthys olivaceus antimicrobial peptide bactericidal |
| DOI | 10.3390/ijms232112722 |
| 通讯作者 | Sun, Li(lsun@qdio.ac.cn) |
| 英文摘要 | NK-lysin (NKL) is a family of antimicrobial proteins with an important role in innate and adaptive immunity. In this study, a non-canonical NK-lysin (NKLnc) was identified in the Japanese flounder (Paralichthys olivaceus), which shares low sequence identities (15.8-20.6%) with previously reported fish NKLs and was phylogenetically separated from the canonical NKLs in teleost. NKLnc expression was upregulated in flounder tissues during bacterial infection, and interference with NKLnc expression impaired the ability of flounder cells to eliminate invading bacteria. When expressed in Escherichia coli, NKLnc was detrimental to the host cells. P35, a peptide derived from the saposin B domain (SapB) of NKLnc, bound major bacterial surface molecules and killed both Gram-negative and Gram-positive bacteria by inflicting damage to bacterial cell structure and genomic DNA. The bactericidal activity, but not the bacteria-binding capacity, of P35 required the structural integrity of the alpha 2/3 helices in SapB. Furthermore, P35 induced the migration of flounder peripheral blood leukocytes, inhibited bacterial dissemination in fish tissues, and facilitated fish survival after bacterial challenge. Together our study reveals that NKLnc plays an important part in flounder immune defense, and that NKLnc peptide exerts an antimicrobial effect via multiple mechanisms by targeting both bacteria and fish cells. |
| 资助项目 | National Key Research and Development Project of China[2018YFD0900500] ; Taishan Scholar Program of Shandong Province |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000881196400001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/180416]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Li |
| 作者单位 | 1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Hang,Yuan, Zihao,Sun, Li. A Non-Canonical Teleost NK-Lysin: Antimicrobial Activity via Multiple Mechanisms[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2022,23(21):15. |
| APA | Xu, Hang,Yuan, Zihao,&Sun, Li.(2022).A Non-Canonical Teleost NK-Lysin: Antimicrobial Activity via Multiple Mechanisms.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,23(21),15. |
| MLA | Xu, Hang,et al."A Non-Canonical Teleost NK-Lysin: Antimicrobial Activity via Multiple Mechanisms".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23.21(2022):15. |
入库方式： OAI收割
来源：海洋研究所
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