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The synthesis, characterization and immunological activity of mucopolysaccharide-quaternized chitosan nanoparticles

文献类型：期刊论文

作者Xu, Chaojie1,2,3,4; Xing, Ronge1,2,3; Liu, Song1,2,3; Qin, Yukun1,2,3; Li, Kecheng1,2,3; Yu, Huahua1,2,3; Li, Pengcheng1,2,3
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2022-11-01
卷号220页码:258-266
ISSN号0141-8130
关键词Mucopolysaccharide Quaternized chitosan Nanoparticles DCS cells Immunocompetence
DOI10.1016/j.ijbiomac.2022.08.069
通讯作者Xing, Ronge(xingronge@qdio.ac.cn) ; Li, Pengcheng(pcli@qdio.ac.cn)
英文摘要In this study, nanoparticles were prepared by using positively charged quaternized chitosan and negatively charged mucopolysaccharide such as chondroitin sulfate, heparin and hyaluronic acid. The nanoparticles have a stable nanostructure with particle size in 336.2-424.5 nm, potential in 18.5-31.1 mV and polydispersity index PDI of 0.172-0.335. Moreover, their encapsulation efficiency was 68.77 % and 64.89 %, and they have low endotoxin and good stability. It can significantly promote the expression of IL-6, TNF-alpha, and IL-1 beta of DCS cells. Moreover, the in vivo immune activity of heparin-quaternized chitosan-OVA nanoparticles against BALB/C mice was showed that, the nanoparticles could significantly promote the secretion of immunoglobulins in mice including IgG1 and IgG2. And nanoparticle also can promote the production of immune factors. Meanwhile, the expression of immune factor genes was also elevated. Furthermore, the results of tissue section experiments showed that the nanoparticles are safety of the body.
资助项目Key deployment projects of Marine Science Research Center of Chinese Academy of Sciences[COMS2020J04]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
出版者ELSEVIER
WOS记录号WOS:000862931600008
源URL[http://ir.qdio.ac.cn/handle/337002/180569]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Xing, Ronge; Li, Pengcheng
作者单位1.Chinese Acad Sci, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Drugs & Bioprod, 1 Wenhai Rd, Qingdao 266237, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Xu, Chaojie,Xing, Ronge,Liu, Song,et al. The synthesis, characterization and immunological activity of mucopolysaccharide-quaternized chitosan nanoparticles[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2022,220:258-266.
APA Xu, Chaojie.,Xing, Ronge.,Liu, Song.,Qin, Yukun.,Li, Kecheng.,...&Li, Pengcheng.(2022).The synthesis, characterization and immunological activity of mucopolysaccharide-quaternized chitosan nanoparticles.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,220,258-266.
MLA Xu, Chaojie,et al."The synthesis, characterization and immunological activity of mucopolysaccharide-quaternized chitosan nanoparticles".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 220(2022):258-266.

入库方式： OAI收割

来源：海洋研究所

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