Cryptochrome 1 is involved in anti-bacterial immunity in Japanese flounder (Paralichthys olivaceus)
文献类型：期刊论文
| 作者 | Zhang, Peng1,2,3 ; Sun, Boguang1,2,4
|
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2022-12-15 |
| 卷号 | 561页码:11 |
| 关键词 | Paralichthys olivaceus Circadian protein Cryptochrome 1 Immune response Bacterial infection |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2022.738675 |
| 通讯作者 | Sun, Boguang(sunboguang@qdio.ac.cn) |
| 英文摘要 | Cryptochrome is a pivotal protein of the core regulatory machinery in circadian clock and participates in various biological processes including immunity. In fish, the immunological functionality of Cryptochrome is unclear. In this study, we profiled the expression pattern of Japanese flounder cryptochrome 1 (named PoCry1) and inves-tigated its involvement in the immune response during bacterial infection. PoCry1 is approximately 75% iden-tical in amino acid sequence to mammalian Cryptochrome 1 and possesses the conserved DNA photolyase domain and FAD binding domain. Its transcription was detected in 10 flounder tissues and was reduced upon Vibrio harveyi challenge. The transcription of PoCry1 exhibited apparent rhythmic oscillation upon the light actuation of a day-night cycle in different tissues. The knockdown of PoCry1 resulted in significant augment of inflammatory cytokine expression. During bacterial infection, PoCry1 silencing led to increase in cytokine expression and decrease in bacterial dissemination, which could be neutralized by a specific PKA inhibitor, suggesting PoCry1 is likely able to negatively modulate the immune response to bacterial infection through PKA associated pathways.Taken together, our results revealed that PoCry1 is circadian oscillated in transcription upon rhythmic light stimulation and involved in anti-infection immunity, thus enlightening that light condition as a circadian zeit-geber could be exploited to strength fish immune system and benefit disease control in aquaculture. |
| 资助项目 | National Key Research and Development Program of China[2018YFD0900500] ; National Natural Science Foundation of China[41876170] ; Taishan Scholar Program of Shandong Province |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000868091100001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/180620]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Sun, Boguang |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Peng,Sun, Boguang. Cryptochrome 1 is involved in anti-bacterial immunity in Japanese flounder (Paralichthys olivaceus)[J]. AQUACULTURE,2022,561:11. |
| APA | Zhang, Peng,&Sun, Boguang.(2022).Cryptochrome 1 is involved in anti-bacterial immunity in Japanese flounder (Paralichthys olivaceus).AQUACULTURE,561,11. |
| MLA | Zhang, Peng,et al."Cryptochrome 1 is involved in anti-bacterial immunity in Japanese flounder (Paralichthys olivaceus)".AQUACULTURE 561(2022):11. |
入库方式： OAI收割
来源：海洋研究所
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