Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2
文献类型：期刊论文
| 作者 | Geng, Lihua1,2; Zhang, Quanbin1,2; Suo, Qishan1,2,3; Wang, Jing1,2 ; Wang, Yingxia4; Wang, Cong5; Wu, Ning1,2,6
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| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2023 |
| 卷号 | 299页码:9 |
| ISSN号 | 0144-8617 |
| 关键词 | Pyropia yezoensis Oligo-porphyran Oligosaccharide SARS-CoV-2 SPR Pseudovirus test |
| DOI | 10.1016/j.carbpol.2022.120173 |
| 通讯作者 | Wu, Ning(wuning@qdio.ac.cn) |
| 英文摘要 | COVID-19 caused by SARS-CoV-2 has spread around the world at an unprecedented rate. A more homogeneous oligo-porphyran with mean molecular weight of 2.1 kD, named OP145, was separated from Pyropia yezoensis. NMR analysis showed OP145 was mainly composed of -> 3)-beta-D-Gal-(1 -> 4)-alpha-L-Gal (6S) repeating units with few replacement of 3,6-anhydride, and the molar ratio was 1:0.85:0.11. MALDI-TOF MS revealed OP145 contained mainly tetrasulfate-oligogalactan with Dp range from 4 to 10 and with no more than two 3,6-anhydro-alpha-L-Gal replacement. The inhibitory activity of OP145 against SARS-CoV-2 was investigated in vitro and in silico. OP145 could bind to Spike glycoprotein (S-protein) through SPR result, and pseudovirus tests confirmed that OP145 could inhibite the infection with an EC50 of 37.52 mu g/mL. Molecular docking simulated the interaction between the main component of OP145 and S-protein. All the results indicated that OP145 had the potency to treat and prevent COVID-19. |
| 资助项目 | National Key Research and Development program of China[2019YFD0900705] ; Shandong Provincial Natural Science Foundation[ZR2019BD053] ; Science and Technology program of Nantong[MS12021037] ; Special Open Fund of Laboratory for Marine Biology and Biotechnology ; Qingdao Pilot National Laboratory for Marine Science and Technology[OF2020NO02] |
| WOS研究方向 | Chemistry ; Polymer Science |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCI LTD |
| WOS记录号 | WOS:000866652600005 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/180647]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wu, Ning |
| 作者单位 | 1.Inst Oceanol, Chinese Acad Sci, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Publ Technol Serv Ctr, Ctr Ocean Mega Sci, Qingdao, Peoples R China 5.Ocean Univ China, Sch Med & Pharm, Qingdao, Peoples R China 6.Nantong Zhongke Marine Sci & Technol Res & Dev Ctr, Nantong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Geng, Lihua,Zhang, Quanbin,Suo, Qishan,et al. Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2[J]. CARBOHYDRATE POLYMERS,2023,299:9. |
| APA | Geng, Lihua.,Zhang, Quanbin.,Suo, Qishan.,Wang, Jing.,Wang, Yingxia.,...&Wu, Ning.(2023).Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2.CARBOHYDRATE POLYMERS,299,9. |
| MLA | Geng, Lihua,et al."Inhibitory activity of a sulfated oligo-porphyran from Pyropia yezoensis against SARS-CoV-2".CARBOHYDRATE POLYMERS 299(2023):9. |
入库方式： OAI收割
来源：海洋研究所
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