A pattern recognition receptor ficolin from Portunus trituberculatus (Ptficolin) regulating immune defense and hemolymph coagulation
文献类型：期刊论文
| 作者 | Liu, Yuan1,2,3,4,6,7; Zhang, Ao1,2,5; Guo, Na1,2,5; Hao, Qiang1,2,5; Li, Fuhua1,2,3,4,6 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2022-11-30 |
| 卷号 | 221页码:558-572 |
| ISSN号 | 0141-8130 |
| 关键词 | Ficolin Portunus trituberculatus Pattern recognition receptor Innate immunity Hemolymph clotting |
| DOI | 10.1016/j.ijbiomac.2022.09.041 |
| 通讯作者 | Liu, Yuan(liuyuan@qdio.ac.cn) |
| 英文摘要 | Ficolins, belonging to the fibrinogen-related protein superfamily, are important pattern recognition receptors in innate immunity. Here, a ficolin gene Ptficolin was characterized from the swimming crab Portunus trituberculatus. The completed cDNA sequence of Ptficolin encoded a signal peptide, a coiled-coil region and a fibrinogen -like domain but without the typical collagen region of vertebrate ficolins. Ptficolin showed higher expression in stomach and hepatopancreas, and presented a time-dependent response after pathogen challenge and injury stimulation. The recombinant Ptficolin (rPtficolin) could bind to various PAMPs and microorganisms, and agglutinate microorganisms and rabbit erythrocytes in a Ca2+-dependent manner, with strong binding ability to N-acetyl sugars. Meanwhile, rPtficolin promoted the hemocyte phagocytosis and clearance activity of Vibrio, while Ptficolin knockdown impaired the bacterial phagocytosis and clearance ability, suggesting the opsonin activity of Ptficolin. Knockdown of Ptficolin could downregulate the transcription of most complement-like genes and AMPs, but enhance the expression of most proPO system-related genes and key genes of Toll, IMD and JNK pathways. Moreover, knockdown of Ptficolin led to the increased hemolymph clotting time and the decreased expression of clotting-related genes. Our results indicate that Ptficolin could recognize and eliminate invading pathogens, and might be a prominent component in hemolymph coagulation of crab. |
| 资助项目 | National Key R & D Program of China[2018YFD0901304] ; National Natural Science Foundation of China[41776159] ; China Agriculture Research System - 48[CARS -48] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000859383200003 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/180681]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Yuan |
| 作者单位 | 1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 5.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Chinese Acad Sci, Inst Oceanol, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yuan,Zhang, Ao,Guo, Na,et al. A pattern recognition receptor ficolin from Portunus trituberculatus (Ptficolin) regulating immune defense and hemolymph coagulation[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2022,221:558-572. |
| APA | Liu, Yuan,Zhang, Ao,Guo, Na,Hao, Qiang,&Li, Fuhua.(2022).A pattern recognition receptor ficolin from Portunus trituberculatus (Ptficolin) regulating immune defense and hemolymph coagulation.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,221,558-572. |
| MLA | Liu, Yuan,et al."A pattern recognition receptor ficolin from Portunus trituberculatus (Ptficolin) regulating immune defense and hemolymph coagulation".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 221(2022):558-572. |
入库方式： OAI收割
来源：海洋研究所
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