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Knockout of a diatom cryptochrome by CRISPR/Cas9 causes an increase in light-harvesting protein levels and accumulation of fucoxanthin

文献类型：期刊论文

作者Yang, Wenting1,2,3; Zhou, Lu2,3,4; Wang, Jing2; Wang, Lijun2,3; Gao, Shan2,3; Wang, Guangce2,3
刊名ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
出版日期2022-07-01
卷号66页码:9
ISSN号2211-9264
关键词Fucoxanthin Cryptochrome Phaeodactylum tricornutum
DOI10.1016/j.algal.2022.102822
通讯作者Gao, Shan(shangao@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要Diatoms are a promising source of fucoxanthin, a carotenoid that has many beneficial effects in human health. However, the gene encoding the last step of the fucoxanthin biosynthesis pathway in diatoms remains uniden-tified, and the molecular regulatory mechanisms are unknown. In this study, we show that cryptochrome CryP, a blue light-sensitive protein in the diatom Phaeodactylum tricornutum regulates fucoxanthin biosynthesis. CryP transcripts showed a diurnal rhythmic expression pattern; however, CryP protein showed high stability under different photoperiods. Furthermore, CryP not only regulates the expression of genes encoding carotenoid biosynthesis, but also affects the level of fucoxanthin chlorophyll a/c-binding proteins (FCPs). Upon knockout of the CryP gene, no change in cell morphology or growth was observed. However, both fucoxanthin content and FCP levels in P. tricornutum knockout lines increased greatly compared with the wild type. Our study is the first to report cryptochrome knockout in diatoms, which opens the possibility of fucoxanthin production through genome editing in P. tricornutum targeting known crucial steps.
资助项目National Key Research and Development Program of China[2018YFD0901500] ; National Natural Science Foundation of China[41976097] ; National Natural Science Foundation of China[41876163] ; Major Scientific and Technological Innovation Projects in Shandong Province[2019JZZY010815] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; Key Deployment Project of the Centre for Ocean Mega-Research of Science ; Chinese Academy of Sciences[COMS2019Q02] ; China Agriculture Research System of MOF and MARA[CARS-50]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
出版者ELSEVIER
WOS记录号WOS:000861083100005
源URL[http://ir.qdio.ac.cn/handle/337002/180717]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Gao, Shan; Wang, Guangce
作者单位1.Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Key Lab Expt Marine Biol, Qingdao, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
4.Univ Chinese Acad Sci, Coll Earth Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Wenting,Zhou, Lu,Wang, Jing,et al. Knockout of a diatom cryptochrome by CRISPR/Cas9 causes an increase in light-harvesting protein levels and accumulation of fucoxanthin[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2022,66:9.
APA Yang, Wenting,Zhou, Lu,Wang, Jing,Wang, Lijun,Gao, Shan,&Wang, Guangce.(2022).Knockout of a diatom cryptochrome by CRISPR/Cas9 causes an increase in light-harvesting protein levels and accumulation of fucoxanthin.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,66,9.
MLA Yang, Wenting,et al."Knockout of a diatom cryptochrome by CRISPR/Cas9 causes an increase in light-harvesting protein levels and accumulation of fucoxanthin".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 66(2022):9.

入库方式： OAI收割

来源：海洋研究所

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