Genetic Diversity and Geographical Distribution of the Red Tide Species Coscinodiscus granii Revealed Using a High-Resolution Molecular Marker
文献类型：期刊论文
| 作者 | Huang, Hailong1,2,3; Xu, Qing2,3,4; Song, Huiyin2,3,4; Chen, Nansheng2,3,4,5 |
| 刊名 | MICROORGANISMS
 |
| 出版日期 | 2022-10-01 |
| 卷号 | 10期号:10页码:14 |
| 关键词 | red tide species Coscinodiscus granii mitochondrial genomes (mtDNAs) molecular marker genetic diversity |
| DOI | 10.3390/microorganisms10102028 |
| 通讯作者 | Chen, Nansheng(chenn@qdio.ac.cn) |
| 英文摘要 | Diatoms are responsible for approximately 40% of the global primary photosynthetic production and account for up to 20% of global carbon fixation. Coscinodiscus granii is a red tide forming species of the phylum Bacillariophyta that has been detected in a wide range of coastal regions, suggesting the possibility of the existence of high genetic diversity with differential adaptation. Common molecular markers including 18S rDNA, 16S rDNA, ITS, cox1, and rbcL do not provide sufficient resolution for distinguishing intra-species genetic diversity, hindering in-depth research on intra-species genetic diversity and their spatial and temporal dynamics. In this project, we aimed to develop molecular markers with high resolution and specificity for C. granii, attempting to identify different taxa of this species, which will set up a stage for subsequent functional assays. Comparative genomics analysis of the mtDNAs of C. granii strains identified a genomic region with high genomic variations, which was used to guide the development of a molecular marker with high resolution and high specificity. This new molecular marker, which was named cgmt1 (C. granii mitochondrial 1), was 376 bp in size and differentiated C. granii samples collected in coastal regions of China into three different clades. Preliminary analysis of field samples collected in various coastal regions in China revealed that C. granii clades were almost exclusively found in the Bohai Sea and the north Yellow Sea. This newly developed molecular marker cgmt1 could be used for tracking intra-species genetic diversity and biogeographic distribution of C. granii in different ecosystems. |
| 资助项目 | Natural Science Foundation of China[42176162] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDB42000000] ; Chinese Academy of Sciences Pioneer Hundred Talents Program ; Taishan Scholar Project Special Fund ; Qingdao Innovation and Creation Plan[19-3-2-16-zhc] ; Post-doctoral Program of Qingdao[E0KY061] ; Natural Science Foundation of Shandong Province, China[ZR2020QD095] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000873129200001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/180473]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Chen, Nansheng |
| 作者单位 | 1.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China 5.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada |
| 推荐引用方式 GB/T 7714 | Huang, Hailong,Xu, Qing,Song, Huiyin,et al. Genetic Diversity and Geographical Distribution of the Red Tide Species Coscinodiscus granii Revealed Using a High-Resolution Molecular Marker[J]. MICROORGANISMS,2022,10(10):14. |
| APA | Huang, Hailong,Xu, Qing,Song, Huiyin,&Chen, Nansheng.(2022).Genetic Diversity and Geographical Distribution of the Red Tide Species Coscinodiscus granii Revealed Using a High-Resolution Molecular Marker.MICROORGANISMS,10(10),14. |
| MLA | Huang, Hailong,et al."Genetic Diversity and Geographical Distribution of the Red Tide Species Coscinodiscus granii Revealed Using a High-Resolution Molecular Marker".MICROORGANISMS 10.10(2022):14. |
入库方式： OAI收割
来源：海洋研究所
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