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Bacterial DNA analysis based on target aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction

文献类型：期刊论文

作者Zhou, Yanan1,2,3,4; Zeng, Yan1,2; Zhang, Dun1,2; Qi, Peng1,2; Wang, Yingwen1,2,3,4; Xin, Yue1,2,3,4; Sun, Yan1,2
刊名SENSORS AND ACTUATORS B-CHEMICAL
出版日期2023
卷号374页码:7
关键词Bacterial DNA DNA silver nanocluster Three-way DNA junction
DOI10.1016/j.snb.2022.132749
通讯作者Zeng, Yan(zengyan@qdio.ac.cn) ; Zhang, Dun(zhangdun@qdio.ac.cn)
英文摘要We have constructed a simple, enzyme-free and label-free detection method for bacterial DNA. First, bacterial DNA (T) is designed as a trigger to make the designed metastable hairpin probes P1, P2, P3 (carrying DNA silver nanoclusters sequences) autonomously cross-open to achieve catalytic self-assembly, inducing a three-way DNA junction formation through the catalyzed hairpin self-assembly (CHA) mode. Then, G-rich sequences are intro-duced to form DNA silver nanoclusters G quadruplex (DNA-AgNCs-G quadruplex), and a strong fluorescence signal can be collected under the action of silver nitrate and sodium borohydride solutions. Through the fluo-rescent reporter of DNA silver nanoclusters (DNA-AgNCs) and the amplification of CHA, we successfully detected target DNA at concentrations as low as 19.95 fM. Compared with the constructed acyclic system, the sensitivity of this cyclic system is improved about three orders of magnitude. Moreover, this recycling system achieves good selectivity for mismatched target DNA and excellent recovery rate in practical samples. Therefore, bacterial DNA analysis based on target-aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction is successfully developed, promising its great application in biological sensing.
资助项目National Natural Science Foundation of China[41906037] ; National Natural Science Foundation of China[41876101] ; Nantong Scientific Plan Foundation[JC2020090] ; Nantong Scientific Plan Foundation[JC2021054] ; Wenzhou Key Laboratory of Sanitary Microbiology[ZD202003KF01] ; Shandong Provincial Marine Science and Technology Projects
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种英语
WOS记录号WOS:000882029800004
出版者ELSEVIER SCIENCE SA
源URL[http://ir.qdio.ac.cn/handle/337002/180280]  
专题海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者Zeng, Yan; Zhang, Dun
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714		 Zhou, Yanan,Zeng, Yan,Zhang, Dun,et al. Bacterial DNA analysis based on target aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction[J]. SENSORS AND ACTUATORS B-CHEMICAL,2023,374:7.
APA Zhou, Yanan.,Zeng, Yan.,Zhang, Dun.,Qi, Peng.,Wang, Yingwen.,...&Sun, Yan.(2023).Bacterial DNA analysis based on target aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction.SENSORS AND ACTUATORS B-CHEMICAL,374,7.
MLA Zhou, Yanan,et al."Bacterial DNA analysis based on target aided self-assembly cycle amplification coupled with DNA-AgNCs/three-way DNA junction".SENSORS AND ACTUATORS B-CHEMICAL 374(2023):7.

入库方式： OAI收割

来源：海洋研究所

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