Three-dimensional ordered macroporous MOF-based smart gating membrane with size screening effect and aptamer specificity for highly efficient thrombin isolation
文献类型:期刊论文
作者 | Lin, Yanna1; Xu, Yulong1; Xing, Yanzhi1; Liu, Nazhen2; Chen, Xuwei1 |
刊名 | JOURNAL OF MEMBRANE SCIENCE |
出版日期 | 2023-01-05 |
卷号 | 665页码:13 |
ISSN号 | 0376-7388 |
关键词 | Three-dimensional ordered macroporous metal -organic framework Membrane separation Thrombin Aptamer Size screening |
DOI | 10.1016/j.memsci.2022.121132 |
通讯作者 | Liu, Nazhen(liunazhen@qdio.ac.cn) ; Chen, Xuwei(chenxuwei@mail.neu.edu.cn) |
英文摘要 | Membrane separation is an efficient technique for the separation and extraction of biomolecules from complex matrices. Herein, a thrombin aptamer-functionalized temperature-responsive smart gating metal-organic framework (MOF) membrane was fabricated by three-dimensional ordered macroporous ZIF-8 (3DOM ZIF-8) and copolymer of sodium alginate grafted with poly(N-isopropylacrylamide) (SA-PNIPAM) for thrombin (TB) isolation. The SA-PNIPAM on the exterior surface of 3DOM MOFs endows the membrane with favorable protein penetration selectivity via temperature-controlled size-screening effect, and the aptamer functionalized on the interior macropore provides excellent thrombin capturing specificity. The separation factor of the smart gating membrane ranges from 13.88 to 49.22 for mixtures of TB with other protein species, and TB enrichment factor is high up to 95.97 under a TB/HSA mass ratio of 1:1000. The aptamer functionalized smart gating membrane is applied to the selective isolation of TB from the human serum. Fibrin production tests demonstrate that the biological activity of the isolated TB is well maintained and suitable for ensuing applications. Moreover, this 3DOM MOF-based smarting gating membrane exhibits high fouling-resistance performance. |
资助项目 | LiaoNing Science and Technology Development Foundation Guided by Central Government[2022JH6/100100024] ; Fundamental Research Funds for the Cen- tral Universities[N2005027] ; National Natural Science Foundation of China[22074011] |
WOS研究方向 | Engineering ; Polymer Science |
语种 | 英语 |
出版者 | ELSEVIER |
WOS记录号 | WOS:000885059500002 |
源URL | [http://ir.qdio.ac.cn/handle/337002/180301] |
专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
通讯作者 | Liu, Nazhen; Chen, Xuwei |
作者单位 | 1.Northeastern Univ, Coll Sci, Res Ctr Analyt Sci, Dept Chem, Box 332, Shenyang 110819, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China |
推荐引用方式 GB/T 7714 | Lin, Yanna,Xu, Yulong,Xing, Yanzhi,et al. Three-dimensional ordered macroporous MOF-based smart gating membrane with size screening effect and aptamer specificity for highly efficient thrombin isolation[J]. JOURNAL OF MEMBRANE SCIENCE,2023,665:13. |
APA | Lin, Yanna,Xu, Yulong,Xing, Yanzhi,Liu, Nazhen,&Chen, Xuwei.(2023).Three-dimensional ordered macroporous MOF-based smart gating membrane with size screening effect and aptamer specificity for highly efficient thrombin isolation.JOURNAL OF MEMBRANE SCIENCE,665,13. |
MLA | Lin, Yanna,et al."Three-dimensional ordered macroporous MOF-based smart gating membrane with size screening effect and aptamer specificity for highly efficient thrombin isolation".JOURNAL OF MEMBRANE SCIENCE 665(2023):13. |
入库方式: OAI收割
来源:海洋研究所
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