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Simultaneous ultrasensitive ADP and ATP quantification based on CRISPR/ Cas12a integrated ZIF-90@Ag3AuS2@Fe3O4 nanocomposites

文献类型：期刊论文

作者Wang, Yingwen1,2,3; Zhang, Dun1,2,3; Zeng, Yan1,3; Sun, Yan1,3; Qi, Peng1,2,3
刊名BIOSENSORS & BIOELECTRONICS
出版日期2022-12-15
卷号218页码:10
关键词Simultaneous detection Ultrasensitive quantification CRISPR Cas12a ZIF-90@Ag3AuS2@Fe3O4 ADP and ATP
ISSN号0956-5663
DOI10.1016/j.bios.2022.114784
通讯作者Zhang, Dun(zhangdun@qdio.ac.cn) ; Qi, Peng(qipeng@qdio.ac.cn)
英文摘要ADP/ATP ratio is a sensitive indicator of changes in cellular energy status and is important for regulating cell signaling activities. Ultrasensitive quantification of ADP and ATP concentration in a single system is in great demand for bioanalysis and early disease diagnosis. Hence, a target-regulated luminous nanoplatform based on clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a integrated zeolite imidazolate framework (ZIF-90)@Ag3AuS2@Fe3O4 nanocomposites was established for the simultaneous detection of ADP and ATP. This simultaneous and ultrasensitive quantification nanoplatform (dsDNA-ZIF-90@Ag3AuS2@Fe3O4) composed an ADP sensitive module based on the aptamer-activated CRISPR/Cas12a and an ATP responsive module based on ATP-triggered ZIF-90 decomposition and quencher loading release. The binding and signaling processes of the different nucleotides were independent, and there was no interference between the two modules. Utilizing the high specific binding and strong signal amplification of this method, limits of detection as low as 0.022 and 0.079 nM were obtained within 30 min for ADP and ATP, respectively. Moreover, the proposed biosensor exhibited high accuracy, specificity, and excellent recovery in serum samples and bacterial biofilms. Therefore, the dsDNA-ZIF-90@Ag3AuS2@Fe3O4-based nanoplatform provides a promising method for ultrasensitive dual-mode quantification of ADP and ATP in the same system, possessing great potential for bioanalysis and early disease diagnosis.
资助项目National Natural Science Foundation of China ; Nantong Scientific Plan Foundation ; Open fund of Wenzhou Key Laboratory of Sanitary Microbiology ; Shandong Provincial Marine Science and Technology Projects ; [41876101and 41906037] ; [JC2021054] ; [JC2020090] ; [ZD202003KF01]
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种英语
WOS记录号WOS:000876432600005
出版者ELSEVIER ADVANCED TECHNOLOGY
源URL[http://ir.qdio.ac.cn/handle/337002/180503]  
专题海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者Zhang, Dun; Qi, Peng
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Yingwen,Zhang, Dun,Zeng, Yan,et al. Simultaneous ultrasensitive ADP and ATP quantification based on CRISPR/ Cas12a integrated ZIF-90@Ag3AuS2@Fe3O4 nanocomposites[J]. BIOSENSORS & BIOELECTRONICS,2022,218:10.
APA Wang, Yingwen,Zhang, Dun,Zeng, Yan,Sun, Yan,&Qi, Peng.(2022).Simultaneous ultrasensitive ADP and ATP quantification based on CRISPR/ Cas12a integrated ZIF-90@Ag3AuS2@Fe3O4 nanocomposites.BIOSENSORS & BIOELECTRONICS,218,10.
MLA Wang, Yingwen,et al."Simultaneous ultrasensitive ADP and ATP quantification based on CRISPR/ Cas12a integrated ZIF-90@Ag3AuS2@Fe3O4 nanocomposites".BIOSENSORS & BIOELECTRONICS 218(2022):10.

入库方式： OAI收割

来源：海洋研究所

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