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Global landscape of lysine acylomes in Bacillus subtilis

文献类型：期刊论文

作者Zhang, Mingya3,4; Liu, TianXian3; Wang, Le3,4; Huang, Yuqi3; Fan, Rufeng3; Ma, Ke3,4; Kan, Yunbo2,3; Tan, Minjia1,2,3,4; Xu, Jun-Yu2,3
刊名JOURNAL OF PROTEOMICS
出版日期2023-01-16
卷号271页码:12
ISSN号1874-3919
关键词Bacillus subtilis Lysine acetylation Lysine propionylation Lysine succinylation Lysine malonylation Mass spectrometry
DOI10.1016/j.jprot.2022.104767
通讯作者Tan, Minjia(mjtan@simm.ac.cn) ; Xu, Jun-Yu(jyxu@simm.ac.cn)
英文摘要Lysine acetylation is a common posttranslational modification that regulates numerous biochemical functions in both eukaryotic and prokaryotic species. In addition, several new non-acetyl acylations are structurally different from lysine acetylation and participate in diverse physiological functions. Here, a comprehensive analysis of several lysine acylomes was performed by combining the high-affinity antibody enrichment with high-resolution LC-MS/MS. In total, we identified 2536 lysine acetylated sites, 4723 propionylated sites, 2150 succinylated sites and 3001 malonylated sites in Bacillus subtilis, respectively. These acylated proteins account for 35.8% of total protein in this bacterium. The four lysine acylomes showed a motif preference for glutamate surrounding the modified lysine residues, and a functional preference for several metabolic pathways, such as carbon metabolism, fatty acid metabolism, and ribosome. In addition, more protein-protein interaction clusters were identified in the propionylated substrates than other three lysine acylomes. In summary, our study presents a global landscape of acylation in the Gram-positive model organism Bacillus and their potential functions in metabolism and physiology.
WOS关键词ESCHERICHIA-COLI ; PROTEIN ACETYLATION ; REVEALS ; PROPIONYLATION ; IDENTIFICATION ; SUCCINYLATION ; MALONYLATION ; INVOLVEMENT ; IMPACT ; COBB
资助项目National Key Research and Development Program of China ; Shanghai Rising-Star Pro-gram ; Natural Science Foundation of China ; Youth Innovation Promotion As-sociation CAS ; [2021YFA0804700] ; [2021YFA0804701] ; [22QA1411100] ; [22225702] ; [31901036] ; [2021276]
WOS研究方向Biochemistry & Molecular Biology
语种英语
出版者ELSEVIER
WOS记录号WOS:000883670700001
源URL[http://119.78.100.183/handle/2S10ELR8/302894]  
专题新药研究国家重点实验室
通讯作者Tan, Minjia; Xu, Jun-Yu
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Zhongshan Inst Drug Discovery, Zhongshan 528400, Guangdong, Peoples R China
2.Jiangsu Ocean Univ, Coll Pharm, Jiangsu Key Lab Marine Pharmaceut Cpd Screening, Lianyungang, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
4.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Jiangsu, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Mingya,Liu, TianXian,Wang, Le,et al. Global landscape of lysine acylomes in Bacillus subtilis[J]. JOURNAL OF PROTEOMICS,2023,271:12.
APA Zhang, Mingya.,Liu, TianXian.,Wang, Le.,Huang, Yuqi.,Fan, Rufeng.,...&Xu, Jun-Yu.(2023).Global landscape of lysine acylomes in Bacillus subtilis.JOURNAL OF PROTEOMICS,271,12.
MLA Zhang, Mingya,et al."Global landscape of lysine acylomes in Bacillus subtilis".JOURNAL OF PROTEOMICS 271(2023):12.

入库方式： OAI收割

来源：上海药物研究所

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