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Protein C-Terminal Tyrosine Conjugation via Recyclable Immobilized BmTYR

文献类型：期刊论文

作者Ji, Xingyu3,4; Zhu, Nanlin2; Ma, Yanjie4; Liu, Jia1,2; Hu, Youhong1,3,4
刊名ACS OMEGA
出版日期2022-10-30
页码8
ISSN号2470-1343
DOI10.1021/acsomega.2c05794
通讯作者Liu, Jia(jia.liu@simm.ac.cn) ; Hu, Youhong(yhhu@simm.ac.cn)
英文摘要Protein modification plays an essential role in biological and pharmaceutical research. Due to the ordinary selectivity and inevitable damage to proteins of chemical synthetic methods, increased efforts were focused on biocatalysts which exhibited high regioselectivity and mild reaction conditions. However, separation of the biocatalysts and modified proteins remained a problem, especially when scaling up. Here, we developed a simple method for site-specific protein modification with a recyclable biocatalyst. The immobilizing tyrosinase (BmTYR) on magnetic beads can oxidize C-terminal tyrosine residues of the target protein to o-quinone, followed by the spontaneous addition of different nucleophiles (e.g., aniline derivatives), resulting in a C-terminal modified protein. Compared to the homogeneous biocatalytic system reported before, this heterogeneous system leads to an easier separation. Furthermore, the solid-phase biocatalyst can be regenerated during separation, providing reusability and lower costs.
WOS关键词DRUG ; BIOCONJUGATION ; SORTASE ; LIGATION ; REAGENTS ; ENZYME
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000879482100001
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/302909]  
专题新药研究国家重点实验室
通讯作者Liu, Jia; Hu, Youhong
作者单位1.Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Mat, State Key Lab Drug Res, Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Ji, Xingyu,Zhu, Nanlin,Ma, Yanjie,et al. Protein C-Terminal Tyrosine Conjugation via Recyclable Immobilized BmTYR[J]. ACS OMEGA,2022:8.
APA Ji, Xingyu,Zhu, Nanlin,Ma, Yanjie,Liu, Jia,&Hu, Youhong.(2022).Protein C-Terminal Tyrosine Conjugation via Recyclable Immobilized BmTYR.ACS OMEGA,8.
MLA Ji, Xingyu,et al."Protein C-Terminal Tyrosine Conjugation via Recyclable Immobilized BmTYR".ACS OMEGA (2022):8.

入库方式： OAI收割

来源：上海药物研究所

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