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A Sensitive and Reversible Labeling Strategy Enables Global Mapping of the Core-Fucosylated Glycoproteome on Cell Surfaces

文献类型：期刊论文

作者Tian, Yinping5; Wang, Yuqiu3,4; Yin, Hongbo2; Luo, Yawen1,5; Wei, Fangyu1,2,5; Zhou, Hu1,4; Wen, Liuqing1,5
刊名ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
出版日期2022-11-09
页码9
关键词Cancer Cell Surfaces Chemoenzymatic Labeling Core Fucosylation Glycoproteomics
ISSN号1433-7851
DOI10.1002/anie.202206802
通讯作者Zhou, Hu(zhouhu@simm.ac.cn) ; Wen, Liuqing(lwen@simm.ac.cn)
英文摘要Core fucosylation, the attachment of alpha 1,6-fucose to the innermost N-acetylglucosamine (GlcNAc) residue of N-glycans, has a strong relationship with tumor growth, invasion, metastasis, prognosis, and immune evasion by regulating many membrane proteins. However, details about the functional mechanism are still largely unknown due to the lack of an effective analytical method to identify cell-surface core-fucosylated glycoproteins, and especially glycosylation sites. Here, we developed a sensitive and reversible labeling strategy for probing core fucosylation, by which core-fucosylated glycoproteins that located on cell-surface were selectively tagged by a biotinylated probe with high sensitivity. The labeled probe can be further broken enzymatically after the capture by affinity resin. The on-bead traceless cleavage allowed the global mapping of core-fucosylated glycoproteins and glycosylation sites by mass spectrometry (MS). The profile of core-fucosylated glycoproteome provides an in-depth understanding of the biological functions of core fucosylation.
WOS关键词CHEMOENZYMATIC SYNTHESIS ; ALPHA(1)-ACID GLYCOPROTEIN ; HIGH-MANNOSE ; E-CADHERIN ; FUT8 ; IDENTIFICATION ; FRAGMENTATION ; GLYCOSYLATION ; EXPRESSION ; CARCINOMA
资助项目Lingang Laboratory[LG-QS-202206-08] ; National Natural Science Foundation of China[22007092] ; Shanghai Sailing Program[20YF1457200] ; National Key Research and Development Program[2020YFA0509000] ; Shanghai Young Excellent Academic Leader Program[20XD1424900] ; Shanghai Municipal Science and Technology Major Project
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000880477900001
出版者WILEY-V C H VERLAG GMBH
源URL[http://119.78.100.183/handle/2S10ELR8/302918]  
专题新药研究国家重点实验室
通讯作者Zhou, Hu; Wen, Liuqing
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China
3.East China Univ Sci & Technol, Sch Bioengn, Shanghai 200237, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Mat Media, State Key Lab Drug Res, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China
5.Chinese Acad Sci, Shanghai Inst Mat Media, Carbohydrate Based Drug Res Ctr, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Tian, Yinping,Wang, Yuqiu,Yin, Hongbo,et al. A Sensitive and Reversible Labeling Strategy Enables Global Mapping of the Core-Fucosylated Glycoproteome on Cell Surfaces[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2022:9.
APA Tian, Yinping.,Wang, Yuqiu.,Yin, Hongbo.,Luo, Yawen.,Wei, Fangyu.,...&Wen, Liuqing.(2022).A Sensitive and Reversible Labeling Strategy Enables Global Mapping of the Core-Fucosylated Glycoproteome on Cell Surfaces.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,9.
MLA Tian, Yinping,et al."A Sensitive and Reversible Labeling Strategy Enables Global Mapping of the Core-Fucosylated Glycoproteome on Cell Surfaces".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2022):9.

入库方式： OAI收割

来源：上海药物研究所

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