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Generation of a TPH2-EGFP reporter cell line for purification and monitoring of human serotonin neurons in vitro and in vivo

文献类型：期刊论文

作者Xu, Ting4; Duan, Jinjin3,4; Li, Yingqi4; Wang, Guanhao4; Li, Shuanqing4; Li, You4; Lu, Wenting4; Yan, Xinyi4; Ren, Yixuan4; Guo, Fei2
刊名STEM CELL REPORTS
出版日期2022-10-11
卷号17期号:10页码:2365-2379
ISSN号2213-6711
DOI10.1016/j.stemcr.2022.08.012
通讯作者Cao, Lining(crystalvivining@aliyun.com) ; Lu, Jianfeng(lu.jianfeng@tongji.edu.cn)
英文摘要Generation of serotonin neurons (SNs) from human pluripotent stem cells (hPSCs) provides a promising platform to explore the mech-anisms of serotonin-associated neuropsychiatric disorders. However, neural differentiation always yields heterogeneous cell populations, making it difficult to identify and purify SNs in vitro or track them in vivo following transplantation. Herein, we generated a TPH2-EGFP reporter hPSC line with insertion of EGFP into the endogenous tryptophan hydroxylase 2 (TPH2) locus using CRISPR-Cas9-mediated gene editing technology. This TPH2-reporter, which faithfully indicated TPH2 expression during differentiation, enabled us to obtain purified SNs for subsequent transcriptional analysis and study of pharmacological responses to antidepressants. In addition, the reporter system showed strong EGFP expression to indicate SNs, which enabled us to explore in vitro and ex vivo electrophysiological properties of SNs. In conclusion, this TPH2-EGFP reporter cell line might be of great significance for studies on human SN-related development and differentiation, drug screening, disease modeling, and cell replacement therapies.
WOS关键词TRYPTOPHAN
资助项目National Key Research and Devel- opment Program of China ; National Natural Science Foundation of China ; Fundamental Research Funds for the Central Universities ; [2021YFA1101300] ; [2017YFA0104100] ; [31771644] ; [81801331] ; [31930068]
WOS研究方向Cell Biology
语种英语
WOS记录号WOS:000872115100002
出版者CELL PRESS
源URL[http://119.78.100.183/handle/2S10ELR8/303118]  
专题中国科学院上海药物研究所
通讯作者Cao, Lining; Lu, Jianfeng
作者单位1.Suzhou Inst Tongji Univ, Suzhou 215101, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, Shanghai 201203, Peoples R China
3.Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China
4.Tongji Univ, Shanghai YangZhi Rehabil Hosp, Shanghai Sunshine Rehabil Ctr, Frontier Sci Ctr Stem Cell Res, Shanghai 200092, Peoples R China
推荐引用方式
GB/T 7714		 Xu, Ting,Duan, Jinjin,Li, Yingqi,et al. Generation of a TPH2-EGFP reporter cell line for purification and monitoring of human serotonin neurons in vitro and in vivo[J]. STEM CELL REPORTS,2022,17(10):2365-2379.
APA Xu, Ting.,Duan, Jinjin.,Li, Yingqi.,Wang, Guanhao.,Li, Shuanqing.,...&Lu, Jianfeng.(2022).Generation of a TPH2-EGFP reporter cell line for purification and monitoring of human serotonin neurons in vitro and in vivo.STEM CELL REPORTS,17(10),2365-2379.
MLA Xu, Ting,et al."Generation of a TPH2-EGFP reporter cell line for purification and monitoring of human serotonin neurons in vitro and in vivo".STEM CELL REPORTS 17.10(2022):2365-2379.

入库方式： OAI收割

来源：上海药物研究所

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