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Identification of hexosamine biosynthesis pathway as a novel prognostic signature and its correlation with immune infiltration in bladder cancer

文献类型：期刊论文

作者Cui, Yangyan6; Feng, Hanyi4,5; Liu, Jiakuan2,3; Wu, Jiajun3; Zhu, Rujian2; Huang, Ruimin4,5; Yan, Jun1,2,3
刊名FRONTIERS IN MOLECULAR BIOSCIENCES
出版日期2022-09-08
卷号9页码:13
关键词hexosamine biosynthesis pathway bladder cancer prognosis immune infiltration risk score
DOI10.3389/fmolb.2022.1009168
通讯作者Zhu, Rujian(20667@shpdh.org) ; Huang, Ruimin(rmhuang@simm.ac.cn) ; Yan, Jun(yan_jun@fudan.edu.cn)
英文摘要Background: Urinary bladder cancer (UBC) is one of the common urological malignancies, lacking reliable biomarkers to predict clinical outcomes in UBC patients. Thus, it is needed to identify the novel diagnostic/prognostic biomarkers to stratify the high-risk UBC patients. As a shunt pathway of glycolysis, the hexosamine biosynthesis pathway (HBP) has been implicated in carcinogenesis. However, its prognostic value in UBC remains unclear.Methods: The RNA sequencing and mRNA microarray datasets were downloaded from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus databases. The expression levels of five HBP genes were analyzed in normal and UBC samples, and their associations with stage, grade and survival were plotted. The performance of HBP risk group was evaluated by receiver-operating characteristics (ROC) curve. The HBP signature was generated by Gene Set Variation Analysis (GSVA) and its association with clinicopathological parameters and survival were analyzed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were carried out to examine the potential biological functions of HBP using DAVID online tool. The infiltration estimation fraction of immune cells was performed using CIBERSORT-ABS algorithm. Gene set enrichment analysis (GSEA) was used to explore the potential function of HBP in tumor immunoregulation.Results: Four HBP genes were upregulated in UBCs compared to normal tissues in TCGA-BLCA dataset. The upregulation of all five HBP genes was significantly associated with tumor grade and stage of UBC in three independent UBC datasets. The expression of HBP genes predicted poor clinical outcomes in UBC patients in both TCGA-BLCA and GSE13507 datasets. The high-risk group based on HBP genes showed a poor prognosis. Furthermore, HBP signature was positively associated with tumor grade and stage in TCGA-BLCA dataset and with tumor grade, stage, distal metastasis and poor survival in GSE13507 dataset. Interestingly, high-HBP signature group exhibited a high infiltration of immune cells, particularly the macrophage population.Conclusion: We identified that HBP was a promising prognostic biomarker in UBC patients and strongly associated with immune infiltration.
WOS关键词PROSTATE-CANCER ; METABOLIC PATHWAYS ; URINARY-BLADDER
资助项目Shanghai Science and Technology Committee[202140267] ; Shanghai Municipal Science and Technology Major Project ; Project of Shanghai Municipal Health Commission[PY202001] ; Talents Training Program of Pudong Hospital affiliated to Fudan University[Zdxk 2020-04] ; Project of Key Medical Discipline of Pudong Hospital of Fudan University ; [20S11901400]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000875080100001
出版者FRONTIERS MEDIA SA
源URL[http://119.78.100.183/handle/2S10ELR8/303136]  
专题中国科学院上海药物研究所
通讯作者Zhu, Rujian; Huang, Ruimin; Yan, Jun
作者单位1.Nanjing Univ, MOE Key Lab Model Anim Dis Study, Model Anim Res Ctr, Nanjing, Peoples R China
2.Fudan Univ, Shanghai Pudong Hosp, Dept Urol, Pudong Med Ctr, Shanghai, Peoples R China
3.Fudan Univ, Dept Lab Anim Sci, Shanghai, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China
5.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing, Peoples R China
6.Nanjing Univ, Model Anim Res Ctr, Nanjing, Peoples R China
推荐引用方式
GB/T 7714		 Cui, Yangyan,Feng, Hanyi,Liu, Jiakuan,et al. Identification of hexosamine biosynthesis pathway as a novel prognostic signature and its correlation with immune infiltration in bladder cancer[J]. FRONTIERS IN MOLECULAR BIOSCIENCES,2022,9:13.
APA Cui, Yangyan.,Feng, Hanyi.,Liu, Jiakuan.,Wu, Jiajun.,Zhu, Rujian.,...&Yan, Jun.(2022).Identification of hexosamine biosynthesis pathway as a novel prognostic signature and its correlation with immune infiltration in bladder cancer.FRONTIERS IN MOLECULAR BIOSCIENCES,9,13.
MLA Cui, Yangyan,et al."Identification of hexosamine biosynthesis pathway as a novel prognostic signature and its correlation with immune infiltration in bladder cancer".FRONTIERS IN MOLECULAR BIOSCIENCES 9(2022):13.

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来源：上海药物研究所

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