Multi-omics analysis reveals the pathogenesis of db/db mice diabetic kidney disease and the treatment mechanisms of multi-bioactive compounds combination from Salvia miltiorrhiza
文献类型：期刊论文
| 作者 | Xu, Zhuo2; Xiang, Xiang1,2; Su, Shulan2; Zhu, Yue2; Yan, Hui2; Guo, Sheng2; Guo, Jianming2; Shang, Er-Xin2; Qian, Dawei2; Duan, Jin-ao2 |
| 刊名 | FRONTIERS IN PHARMACOLOGY
 |
| 出版日期 | 2022-09-29 |
| 卷号 | 13页码:16 |
| 关键词 | multi-bioactive compounds combination diabetic kidney disease multi-omics Salvia miltiorrhiza PI3K/Akt/FoxO signaling pathways |
| DOI | 10.3389/fphar.2022.987668 |
| 通讯作者 | Su, Shulan(sushulan1974@163.com) ; Duan, Jin-ao(duanja@163.com) |
| 英文摘要 | Diabetic kidney disease (DKD) is a common diabetic complication. Salvia miltiorrhiza has significant therapeutic effects on diabetes complications, although the mechanism remains unclear. Here, biochemical indicators and pathological changes were used to screen out the optimal Salvia miltiorrhiza multi-bioactive compounds combination. Metabolomics, transcriptomics and proteomics were used to explore the pathogenesis of DKD. RT-PCR and parallel reaction monitoring targeted quantitative proteome analysis were utilized to investigate treatment mechanisms of the optimal Salvia miltiorrhiza multi-bioactive compounds combination. The db/db mice showed biochemical abnormalities and renal lesions. The possible metabolic pathways were steroid hormone biosynthesis and sphingolipid metabolism. The 727 differential genes found in transcriptomics were associated with biochemical indicators via gene network to finally screen 11 differential genes, which were mainly key genes of TGF-beta/Smad and PI3K/Akt/FoxO signaling pathways. Salvia miltiorrhiza multi-bioactive compounds combination could significantly regulate the Egr1, Pik3r3 and Col1a1 genes. 11 differentially expressed proteins involved in the two pathways were selected, of which 9 were significantly altered in db/db mice compared to db/m mice. Salvia miltiorrhiza multi-bioactive compounds combination could callback Q9DBM2, S4R1W1, Q91Y97, P47738, A8DUK4, and A2ARV4. In summary, Salvia miltiorrhiza multi-bioactive compounds combination may ameliorate kidney injury in diabetes through regulation of TGF-beta/Smad and PI3K/Akt/FoxO signaling pathways. |
| WOS关键词 | GENE-EXPRESSION ; PATHWAY ; METABOLISM ; ACTIVATION ; INSIGHTS |
| 资助项目 | National Natural Science Foundation of China[81373889] ; National Natural Science Foundation of China[81673533] ; National Natural Science Foundation of China[81803688] ; National Natural Science Foundation of China[81973708] ; Construction Project for Jiangsu Key Laboratory for High Technology Research of TCM Formulae[BM2010576] ; 333 High-level Talents Training Project - Jiangsu Province ; 2018 Chinese Medicine Public Health Service Subvention Special National traditional Chinese Medicine Resource Survey Project[Caishe (2018) 43] ; Open Project of Chinese Materia Medica Double First-Class Discipline of Nanjing University of Chinese Medicine ; 2021 Jiangsu Province Postgraduate Research and Practice Innovation Program[KYCX21_1760] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000868567700001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/303244]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Su, Shulan; Duan, Jin-ao |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, CAS, Shanghai, Peoples R China 2.Nanjing Univ Chinese Med, Jiangsu Collaborat Innovat Ctr Chinese Med Resourc, Natl & Local Collaborat Engn Ctr Chinese Med Resou, Jiangsu Key Lab High Technol Res TCM Formulae, Nanjing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Zhuo,Xiang, Xiang,Su, Shulan,et al. Multi-omics analysis reveals the pathogenesis of db/db mice diabetic kidney disease and the treatment mechanisms of multi-bioactive compounds combination from Salvia miltiorrhiza[J]. FRONTIERS IN PHARMACOLOGY,2022,13:16. |
| APA | Xu, Zhuo.,Xiang, Xiang.,Su, Shulan.,Zhu, Yue.,Yan, Hui.,...&Duan, Jin-ao.(2022).Multi-omics analysis reveals the pathogenesis of db/db mice diabetic kidney disease and the treatment mechanisms of multi-bioactive compounds combination from Salvia miltiorrhiza.FRONTIERS IN PHARMACOLOGY,13,16. |
| MLA | Xu, Zhuo,et al."Multi-omics analysis reveals the pathogenesis of db/db mice diabetic kidney disease and the treatment mechanisms of multi-bioactive compounds combination from Salvia miltiorrhiza".FRONTIERS IN PHARMACOLOGY 13(2022):16. |
入库方式： OAI收割
来源：上海药物研究所
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