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Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum

文献类型：期刊论文

作者Lv, Xiaojing1,5,6; Jiao, Chunli4; Chen, Yuying2; Chen, Lizhu2; Li, Xuan2; Liu, Xiangquan2; Yang, Dinglong3,5,6; Zhao, Jianmin3,5,6
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2022-12-01
卷号131页码:77-83
ISSN号1050-4648
关键词Ruditapes philippinarum Histone Antimicrobial activity Extracellular traps
DOI10.1016/j.fsi.2022.09.068
通讯作者Yang, Dinglong(dlyang@yic.ac.cn)
英文摘要In the present study, a histone H2A (designed as RpH2A) was identified and characterized from clam Ruditapes philippinarum, and its open reading frame (ORF) was of 387 bp encoding a polypeptide of 128 amino acids. The deduced amino acid sequence of RpH2A shared high identities ranging from 57.1% to 96.1% with that of other identified H2A. The mRNA expression of RpH2A was up-regulated significantly after Vibrio anguillarum chal-lenge. The recombinant RpH2A protein (rRpH2A) displayed significantly binding affinity to lipopolysaccharide (LPS) and peptidoglycan (PGN) in vitro, and also exhibited antimicrobial properties against Escherichia coli. In addition, the antimicrobial RpH2A was shown to co-localize with extracellular traps (ETs) released from he-mocytes induced by E. coli, suggesting that RpH2A might contribute to eliminate invading bacteria in clam ETs. Altogether, our data revealed that RpH2A could function as antimicrobial peptides, which might play a crucial role in the immune responses of hemocytes ETs in clams.
WOS关键词MOLECULAR CHARACTERIZATION ; DISEASE VIRUS ; EXPRESSION ; HEMOCYTES ; IMMUNITY ; PROTEIN ; ANTIBODY ; H1
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000876937200001
资助机构Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; Youth Innovation Promotion Association, Chinese Academy of Sciences ; Natural Science Foundation of Shandong Province ; Yantai Science and Technology Development Project
源URL[http://ir.yic.ac.cn/handle/133337/31978]  
专题海岸带生物资源高效利用研究与发展中心
中国科学院牟平海岸带环境综合试验站
通讯作者Yang, Dinglong
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Shandong Marine Resources & Environm Res Inst, Shandong Key Lab Marine Ecol Restorat, Yantai 264006, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
4.Yantai Ctr Food & Drug Control, Yantai 264003, Peoples R China
5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresour, Yantai 264003, Peoples R China
6.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714		 Lv, Xiaojing,Jiao, Chunli,Chen, Yuying,et al. Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2022,131:77-83.
APA Lv, Xiaojing.,Jiao, Chunli.,Chen, Yuying.,Chen, Lizhu.,Li, Xuan.,...&Zhao, Jianmin.(2022).Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,131,77-83.
MLA Lv, Xiaojing,et al."Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 131(2022):77-83.

入库方式： OAI收割

来源：烟台海岸带研究所

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