Exposure to nitrate induces alterations in blood parameter responses, liver immunity, and lipid metabolism in juvenile turbot (Scophthalmus maximus)
文献类型：期刊论文
| 作者 | Yu, Jiachen2,4; Wang, Xingqiang2,4; Qian, Shiyue4; Liu, Pengfei4; Li, Xian1; Li, Jun3 |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2022-10-01 |
| 卷号 | 251页码:11 |
| ISSN号 | 0166-445X |
| 关键词 | Nitrate exposure Plasma immune parameters Lipid metabolism IBRv2 Scophthalmus maximus |
| DOI | 10.1016/j.aquatox.2022.106280 |
| 通讯作者 | Li, Xian(lixian@qdio.ac.cn) ; Li, Jun(junli1964@126.com) |
| 英文摘要 | Nitrate (NO3-) pollution of waterbodies has attracted significant global attention as it poses a serious threat to aquatic organisms and human beings. This study aimed to evaluate the role of NO3-, an end product of biological nitrification processes, in immune status and lipid metabolism to have a comprehensive understanding of its toxic effects on fishes. Therefore, in this work, juvenile turbot (Scophthalmus maximus) were subjected to four nominal concentrations of NO3- (i.e., 0, 50, 200, 400 mg/L of NO3--N) for a 60-day period. The results indicated that increased exposure to NO3- (200 and/or 400 mg/L) enhanced the concentrations of plasma heat shock protein concentrations (HSP70), complement component 3 (C3), complement component 4 (C4), immunoglobulin M (IgM) and lysozyme (LYS), which meant that NO3- caused fluctuations in the plasma immune system. Higher exposure to NO3- (200 and/or 400 mg/L) also caused significant enhancements in plasma glutamic pyruvic transaminase (GPT), as well as glutamic oxaloacetic transaminase (GOT) activity. Furthermore, NO3- exposure resulted in upregulation of liver TNF-alpha, IL-1 beta, HSP70, HSP90, and LYS. Additionally, the results suggested that NO3- exposure caused a certain degree of histological damage and inflammation in the liver and activated the immune defense processes of juvenile turbot. Furthermore, the mRNA expression levels of certain genes associated with lipid metabolism (peroxisome proliferator-activated receptor-alpha [PPAR-alpha], carnitine palmitoyltransferase 1[CPT1], liver X receptor [LXR] together with sterol regulatory element binding protein-1 [SREBP-1]) increased significantly within fish liver exposed to 200/400 mg/L NO3- -N treatments. Finally, the results obtained from the analysis of the integrated biological responses version 2 (IBRv2) also confirmed the toxic effects of NO3- on juvenile turbot. According to these findings, it can be found that NO3- emission in the aquatic environment needs to be strictly controlled, as it may cause immune and lipid metabolism disorders in fish. |
| 资助项目 | National Key Research and Development Program[2018YFD0901204] ; China Agriculture Research System[CARS -47-G21] ; Priority Academic Program Development of Jiangsu Higher Education Institutions[2021JSPAPD010] |
| WOS研究方向 | Marine & Freshwater Biology ; Toxicology |
| 语种 | 英语 |
| 出版者 | ELSEVIER |
| WOS记录号 | WOS:000853356800003 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/180786]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, Xian; Li, Jun |
| 作者单位 | 1.Ocean Univ China, Coll Fisheries, Qingdao 266003, Peoples R China 2.Coinnovat Ctr Jiangsu Marine Bioind Technol, Lianyungang 222005, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China 4.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Biotechnol, Lab Pathol & Immunol Aquat Anim, Lianyungang 222005, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Jiachen,Wang, Xingqiang,Qian, Shiyue,et al. Exposure to nitrate induces alterations in blood parameter responses, liver immunity, and lipid metabolism in juvenile turbot (Scophthalmus maximus)[J]. AQUATIC TOXICOLOGY,2022,251:11. |
| APA | Yu, Jiachen,Wang, Xingqiang,Qian, Shiyue,Liu, Pengfei,Li, Xian,&Li, Jun.(2022).Exposure to nitrate induces alterations in blood parameter responses, liver immunity, and lipid metabolism in juvenile turbot (Scophthalmus maximus).AQUATIC TOXICOLOGY,251,11. |
| MLA | Yu, Jiachen,et al."Exposure to nitrate induces alterations in blood parameter responses, liver immunity, and lipid metabolism in juvenile turbot (Scophthalmus maximus)".AQUATIC TOXICOLOGY 251(2022):11. |
入库方式： OAI收割
来源：海洋研究所
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