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Overexpression of adenosine 5'-monophosphate deaminase increased umami substance-Inosine 5'- mononucleotide and promoted Neopyropia yezoensis quality

文献类型：期刊论文

作者He, Bangxiang2; Zheng, Zhenbing2; Xie, Xiujun2; Wang, Guangce1,2
刊名ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
出版日期2022-06-01
卷号65页码:6
ISSN号2211-9264
关键词Adenosine-5'-monophosphate deaminase Neopyropia yezoensis Umami substance Genetic transformation
DOI10.1016/j.algal.2022.102737
通讯作者Xie, Xiujun(xjxie@qdio.ac.cn) ; Wang, Guangce(gcwang@qdio.ac.cn)
英文摘要Precision molecular breeding technology plays an important role in improving the yield and quality of cultivated marine crops. In this study, we aimed to improve the quality of Neopyropia yezoensis, one of the important economic seaweeds. We constructed overexpression (OE)-, co-suppression (Ci)-, and RNAi (Ri)-type mutants of Nyampd (AMP deaminase of N. yezoensis), which could catalyze the decomposition of AMP into the umami substance inosine 5'- mononucleotide (IMP) through the genetic transformation system of N. yezoensis. Our results showed that the IMP content and growth rate of OE strain were significantly higher than those of wild type. In addition, in knockdown strains (Ci/Ri), a phenomenon similar to genetic compensation in knockout research occurred, i.e., the expression of Nyampd2 (isozyme of Nyampd) was significantly upregulated in Ri/Ci strains, and the IMP content increased in Ci/Ri strains. These results provide important knowledge for precise molecular breeding and genetic transformation research of N. yezoensis.
资助项目National Key Research and Development Program of China[2018YFD0901500] ; National Natural Science Foundation of China[41906105] ; National Natural Science Foundation of China[41876163] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] ; Key Deployment Project of the Centre for Ocean Mega-Research of Science[COMS2019Q02] ; Major Scientific and Technological Innovation Projects in Shandong Province[2019JZZY010815] ; China Agriculture Research System of MOF and MARA[CARS-50] ; Qingdao Postdoctoral Funding[Y9KY161]
WOS研究方向Biotechnology & Applied Microbiology
语种英语
出版者ELSEVIER
WOS记录号WOS:000856083100010
源URL[http://ir.qdio.ac.cn/handle/337002/180807]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Xie, Xiujun; Wang, Guangce
作者单位1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Megasci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 He, Bangxiang,Zheng, Zhenbing,Xie, Xiujun,et al. Overexpression of adenosine 5'-monophosphate deaminase increased umami substance-Inosine 5'- mononucleotide and promoted Neopyropia yezoensis quality[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2022,65:6.
APA He, Bangxiang,Zheng, Zhenbing,Xie, Xiujun,&Wang, Guangce.(2022).Overexpression of adenosine 5'-monophosphate deaminase increased umami substance-Inosine 5'- mononucleotide and promoted Neopyropia yezoensis quality.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,65,6.
MLA He, Bangxiang,et al."Overexpression of adenosine 5'-monophosphate deaminase increased umami substance-Inosine 5'- mononucleotide and promoted Neopyropia yezoensis quality".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 65(2022):6.

入库方式： OAI收割

来源：海洋研究所

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