Rxrs and their partner receptor genes inducing masculinization plausibly mediated by endocrine disruption in Paralichthys olivaceus
文献类型：期刊论文
| 作者 | Zou, Congcong2,3,4 ; Wang, Lijuan3,4; Shu, Chang2,3,4; Tan, Xungang3,4; Wu, Zhihao3,4; Zou, Yuxia3,4; Li, Ze2,3,4; Wang, Guoyu3,4; Song, Zongcheng1; You, Feng3,4
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| 刊名 | JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
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| 出版日期 | 2023-02-01 |
| 卷号 | 226页码:14 |
| ISSN号 | 0960-0760 |
| 关键词 | Retinoid X receptors Steroidogenesis Sex differentiation LG100268 Gonad Flounder |
| DOI | 10.1016/j.jsbmb.2022.106219 |
| 通讯作者 | You, Feng(youfeng@qdio.ac.cn) |
| 英文摘要 | Retinoid X receptors (RXRs) can form homo-or heterodimers with orphan receptors involved in multiple intertwined signaling pathways. However, there is limited study on the formation of sex phenotypes and the regulation of steroidogenesis by RXRs in fish. Here, in Paralichthys olivaceus, we first indicated that PPAR gamma::RXR alpha was predictably a transcription factor for steroidogenesis genes, and Foxl2 and Dmrt1 were also transcription factors for rxrs and their partner receptor genes. When the flounder fry were exposed to LG100268 (LG, RXRs agonist, 50 mg/kg diet), the percentage of males increased from 50% to 71.4%. Before histological differenti-ation of the flounder ovary (3 cm TL) and testis (6 cm TL), the transcripts of rar beta and rar gamma (P < 0.05) were activated, and the steroidogenesis gene Hsd3b1 was down-regulated (P < 0.05). The ratios of testosterone (T)/ 17 beta-estradiol (E2) were all greatly increased (P < 0.05), and the ratio of 11-ketotestosterone (11-KT)/E2 was elevated at 3 cm TL. Moreover, LG was used to treat the cultured gonads in vitro (10 mu M) and the fish with intraperitoneal injection in vivo (12 mg/kg body weight), respectively. LG was able to up-regulate rxr gamma, rar gamma, and ppar delta, and Hsd3b1 was significantly up-regulated (P < 0.05). The ratios of 11-KT/E2 in the culture medium and in the ovaries of the fish were decreased. Furthermore, the recombinant flounder Foxl2 protein was able to significantly down-regulate ppar gamma (P < 0.05) and tr beta (P < 0.01) in the ovaries in vitro, and the result of the Dmrt1 in the testes was opposite to that of the Foxl2, probably indicating a feedback loop between RXRs' partner re-ceptors and Foxl2/Dmrt1. These findings introduce for the first time the mode of action of RXRs on the flounder steroidogenesis and provide important data to learn the potential function of RXRs in fish sex differentiation and the potential role of RXRs in aquatic animals in the presence of water pollutants. |
| 资助项目 | National Key Research and Devel- opment Program of China ; National Natural Science Foundation of China ; National Marine Genetic Resource Center ; [2018YFD0900202] ; [31772834] ; [31702337] ; [31872558] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Endocrinology & Metabolism |
| 语种 | 英语 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| WOS记录号 | WOS:000891988400008 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/180976]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | You, Feng |
| 作者单位 | 1.Shenghang Aquat Sci & Technol Co Ltd, Weihai 264200, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zou, Congcong,Wang, Lijuan,Shu, Chang,et al. Rxrs and their partner receptor genes inducing masculinization plausibly mediated by endocrine disruption in Paralichthys olivaceus[J]. JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY,2023,226:14. |
| APA | Zou, Congcong.,Wang, Lijuan.,Shu, Chang.,Tan, Xungang.,Wu, Zhihao.,...&You, Feng.(2023).Rxrs and their partner receptor genes inducing masculinization plausibly mediated by endocrine disruption in Paralichthys olivaceus.JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY,226,14. |
| MLA | Zou, Congcong,et al."Rxrs and their partner receptor genes inducing masculinization plausibly mediated by endocrine disruption in Paralichthys olivaceus".JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY 226(2023):14. |
入库方式： OAI收割
来源：海洋研究所
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