Dynamic cellular and molecular characteristics of spermatogenesis in the viviparous marine teleost Sebastes schlegelii
文献类型：期刊论文
| 作者 | Wang, Xueying4,5 ; Liu, Qinghua1,4,5; Li, Jun1,4,5; Zhou, Li3,4,5; Wang, Tao2,4,5; Zhao, Ning3,4,5
|
| 刊名 | BIOLOGY OF REPRODUCTION
 |
| 出版日期 | 2022-11-19 |
| 页码 | 15 |
| ISSN号 | 0006-3363 |
| 关键词 | Sebastes schlegelii internal fertilization spermatogenesis spermiogenesis single-cell RNA-seq |
| DOI | 10.1093/biolre/ioac203 |
| 通讯作者 | Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn) |
| 英文摘要 | In S. schlegelii, luteinizing hormone can induce prostanoid production owing to cox upregulation and prostaglandin may be a key pheromone in copulation and sperm maturation. Spermatogenesis is a dynamic cell developmental process that is essential for reproductive success. Vertebrates utilize a variety of reproductive strategies, including sperm diversity, and internal and external fertilization. Research on the cellular and molecular dynamic changes involved in viviparous teleost spermatogenesis, however, is currently lacking. Here, we combined cytohistology, 10 x genomic single-cell RNA-seq, and transcriptome technology to determine the dynamic development characteristics of the spermatogenesis of Sebastes schlegelii. The expressions of lhcgr (Luteinizing hormone/Choriogonadotropin receptor), fshr (follicle-stimulating hormone receptor), ar (androgen receptor), pgr (progesterone receptor), and cox (cyclo-oxygen-ase), as well as the prostaglandin E and F levels peaked during the maturation period, indicating that they were important for sperm maturation and mating. Fifteen clusters were identified based on the 10 x genomic single-cell results. The cell markers of the sub-cluster were identified by their upregulation; piwil, dazl, and dmrt1 were upregulated and identified as spermatogonium markers, and sycp1/3 and spo11 were identified as spermatocyte markers. For S. schlegelii, the sperm head nucleus was elongated (spherical to streamlined in shape), which is a typical characteristic for sperm involved in internal fertilization. We also identified a series of crucial genes associated with spermiogenesis, such as spata6, spag16, kif20a, trip10, and klf10, while kif2c, kifap3, fez2, and spaca6 were found to be involved in nucleus elongation. The results of this study will enrich our cellular and molecular knowledge of spermatogenesis and spermiogenesis in fish that undergo internal fertilization. |
| WOS研究方向 | Reproductive Biology |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS INC |
| WOS记录号 | WOS:000897040200001 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/181016]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Liu, Qinghua; Li, Jun |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao, Peoples R China 3.Univ Chinese Acad Sci, Coll Marine Sci, Beijing, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 5.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xueying,Liu, Qinghua,Li, Jun,et al. Dynamic cellular and molecular characteristics of spermatogenesis in the viviparous marine teleost Sebastes schlegelii[J]. BIOLOGY OF REPRODUCTION,2022:15. |
| APA | Wang, Xueying,Liu, Qinghua,Li, Jun,Zhou, Li,Wang, Tao,&Zhao, Ning.(2022).Dynamic cellular and molecular characteristics of spermatogenesis in the viviparous marine teleost Sebastes schlegelii.BIOLOGY OF REPRODUCTION,15. |
| MLA | Wang, Xueying,et al."Dynamic cellular and molecular characteristics of spermatogenesis in the viviparous marine teleost Sebastes schlegelii".BIOLOGY OF REPRODUCTION (2022):15. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。