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Confocal Raman microscopy for assessing effects of preservation methods on symbiotic deep-sea mussel gills

文献类型：期刊论文

作者He, Wanying4,5,6; Wang, Minxiao2,3,4; Li, Mengna2,3,4; Zhong, Zhaoshan2,3; Chen, Hao2,3; Xi, Shichuan5,6; Luan, Zhendong4,5,6; Li, Chaolun1; Zhang, Xin4,5,6
刊名FRONTIERS IN MARINE SCIENCE
出版日期2022-12-05
卷号9页码:12
关键词confocal Raman microscopy symbiotic deep-sea mussel preservation methods Raman imaging Raman signals
DOI10.3389/fmars.2022.1077595
通讯作者Li, Chaolun(lcl@qdio.ac.cn) ; Zhang, Xin(xzhang@qdio.ac.cn)
英文摘要Confocal Raman microscopy (CRM) is a powerful tool for biological research, which can provide information regarding the composition and distribution of biomolecules in an in situ, label-free, non-destructive manner and with high spatial resolution. Sample preservation is often an unavoidable step, especially for symbiotic deep-sea samples. Moreover, protocols for the preservation of samples for CRM have not been established and specific effects of different preservation methods on biomolecules have not been studied for relevant samples. In this study, we used deep-sea mussel Gigantidas platifrons, an ideal model in the study of deep-sea symbiosis and investigated the effect of four common preservation methods on the results of CRM imaging and signals. The methods included snap-freeze (SF), SF followed by rapid fixation in methanol (SF-MeOH), 2.5% glutaraldehyde and 2% paraformaldehyde fixation (SF-GP), and 4% paraformaldehyde and alcohol fixation (PS-PA). The results of this study indicate that SF was the most effective method for the comprehensive analysis of the biomolecular composition although the sectioning success rate was relatively low. Moreover, SF-MeOH was found to be effective when SF is not sufficient in obtaining good morphology in sections, or when the effect of chemical bonding on the composition of biomolecules upon SF-MeOH can be neglected. Finally, SF-GP and PS-PA were found to be the most effective methods considering the overall morphological observation. However, they were less suitable for metabolic studies. We believe our results can provide guidance for further studies of Raman on symbiotic deep-sea biological samples. It is of great importance for the wide application of Raman technique.
资助项目Strategic Priority Research Program of Chinese Academy of Sciences[XDA22050102] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA19060402] ; Key project of Ocean Research Center, Chinese Academy of Sciences[COMS2020J03] ; National Natural Science Foundation of China[41822604] ; National Natural Science Foundation of China[42076091] ; Young Taishan Scholars Program[tsqn201909158]
WOS研究方向Environmental Sciences & Ecology ; Marine & Freshwater Biology
语种英语
出版者FRONTIERS MEDIA SA
WOS记录号WOS:000899661800001
源URL[http://ir.qdio.ac.cn/handle/337002/181076]  
专题海洋研究所_海洋地质与环境重点实验室
海洋研究所_海洋生态与环境科学重点实验室
通讯作者Li, Chaolun; Zhang, Xin
作者单位1.Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou, Peoples R China
2.Lab Marine Ecol & Environm Sci, Qingdao Natl Lab Marine Sci & Technol, Qingdao, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Chinese Acad Sci CAS Key Lab Marine Ecol & Environ, Ctr Deep Sea Res, Qingdao, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Pilot Lab Marine Sci & Technol, Lab Marine Geol, Qingdao, Peoples R China
6.Chinese Acad Sci, Inst Oceanol, Chinese Acad Sci Key Lab Marine Geol & Environm, Ctr Deep Sea Res, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 He, Wanying,Wang, Minxiao,Li, Mengna,et al. Confocal Raman microscopy for assessing effects of preservation methods on symbiotic deep-sea mussel gills[J]. FRONTIERS IN MARINE SCIENCE,2022,9:12.
APA He, Wanying.,Wang, Minxiao.,Li, Mengna.,Zhong, Zhaoshan.,Chen, Hao.,...&Zhang, Xin.(2022).Confocal Raman microscopy for assessing effects of preservation methods on symbiotic deep-sea mussel gills.FRONTIERS IN MARINE SCIENCE,9,12.
MLA He, Wanying,et al."Confocal Raman microscopy for assessing effects of preservation methods on symbiotic deep-sea mussel gills".FRONTIERS IN MARINE SCIENCE 9(2022):12.

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来源：海洋研究所

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