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A universal dual-readout viscosity flow sensor based on biotarget-triggered hyaluronidase release from aptamer-capped metal-organic frameworks

文献类型：期刊论文

作者Wang, Yingwen1,2,3; Zhang, Dun1,2,3; Zeng, Yan1,3; Sun, Yan1,3; Qi, Peng1,2,3
刊名SENSORS AND ACTUATORS B-CHEMICAL
出版日期2022-12-01
卷号372页码:8
关键词Dual-readout viscosity flow sensor Aptamer-capped MOF Biotarget triggered release Cytochrome c Hyaluronic acid hydrogel
DOI10.1016/j.snb.2022.132637
通讯作者Zhang, Dun(zhangdun@qdio.ac.cn) ; Qi, Peng(qipeng@qdio.ac.cn)
英文摘要Paper-based sensors are particularly appealing for on-site detection owing to the avoidance of expensive and complex instruments. A dual-readout viscosity flow sensor based on biotarget-triggered hyaluronidase (HAase) release from aptamer-capped metal-organic frameworks (MOFs) was designed for on-site detection. The aptamercapped MOFs (HAase@MOF) were fabricated using UiO-67-NH2 as a nanocarrier for HAase and the doublestranded DNA (dsDNA) as a gatekeeper. The dsDNA structure of HAase@MOF was unlocked to release HAase in the presence of the target; thus, HAase hydrolyzed the hyaluronic acid hydrogel, changing the viscosity and acidity. The reaction solutions were pipetted onto the pH paper strips and the changes in the diffusion distance and the color signal were used to quantify the biotarget concentration. In this context, cytochrome c (cyt c) was selected as the model biotarget. The proposed sensor exhibited excellent specificity and reproducibility for detection of cyt c in human serum and bacterial biofilms. Overall, this study provides a low-cost, rapid workflow, and simple method for biotarget detection with dual-readout signal. Notably, other biotargets could be detected via directly changing the capping dsDNA structure of the MOFs. Thus, the dual-readout viscosity flow sensor possesses great promising in developing commercial products for on-site detection and sensing.
资助项目National Natural Science Foundation of China[41876101] ; National Natural Science Foundation of China[41906037] ; Nantong Scientific Plan Foundation[JC2021054] ; Nantong Scientific Plan Foundation[JC2020090] ; Open fund of Wenzhou Key Laboratory of Sanitary Microbiology[ZD202003KF01] ; Shandong Provincial Marine Science and Technology Projects
WOS研究方向Chemistry ; Electrochemistry ; Instruments & Instrumentation
语种英语
WOS记录号WOS:000888763800006
出版者ELSEVIER SCIENCE SA
源URL[http://ir.qdio.ac.cn/handle/337002/180994]  
专题海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者Zhang, Dun; Qi, Peng
作者单位1.Pilot Natl Lab Marine Sci & Technol Qingdao, Open Studio Marine Corros & Protect, 1 Wenhai Rd, Qingdao 266237, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Yingwen,Zhang, Dun,Zeng, Yan,et al. A universal dual-readout viscosity flow sensor based on biotarget-triggered hyaluronidase release from aptamer-capped metal-organic frameworks[J]. SENSORS AND ACTUATORS B-CHEMICAL,2022,372:8.
APA Wang, Yingwen,Zhang, Dun,Zeng, Yan,Sun, Yan,&Qi, Peng.(2022).A universal dual-readout viscosity flow sensor based on biotarget-triggered hyaluronidase release from aptamer-capped metal-organic frameworks.SENSORS AND ACTUATORS B-CHEMICAL,372,8.
MLA Wang, Yingwen,et al."A universal dual-readout viscosity flow sensor based on biotarget-triggered hyaluronidase release from aptamer-capped metal-organic frameworks".SENSORS AND ACTUATORS B-CHEMICAL 372(2022):8.

入库方式： OAI收割

来源：海洋研究所

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