Multiple airy beams light-sheet fluorescence microscopy
文献类型:期刊论文
作者 | Gu, Shuangyu1,2![]() ![]() ![]() ![]() |
刊名 | FRONTIERS IN PHYSICS
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出版日期 | 2022-12-23 |
卷号 | 10 |
关键词 | light-sheet fluorescence microscopy volumetric imaging non-diffracting beam image contrast field of view |
ISSN号 | 2296-424X |
DOI | 10.3389/fphy.2022.1111023 |
产权排序 | 1 |
英文摘要 | Light-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution. A single Airy beam (SAB) light-sheet can extend the field of view of Light-sheet fluorescence microscopy benefiting from its non-diffracting nature, but at the cost of out-of-focus background and low imaging contrast caused by side lobes illumination. Here, we propose a method to generate a sort of multiple Airy beams (MAB), which are linearly superimposed of multiple single Airy beams with different scale factors. Compared to the SAB light-sheet, the energy of the multiple Airy beams light-sheet is more concentrated on the focal plane of the detection objective, which can improve the imaging contrast and decrease the photodamage effect. Furthermore, we combined the complementary beam subtraction (CBS) strategy to increase the axial resolution, termed as multiple Airy beams-complementary beam subtraction method, which enables the axial resolution of 1.2 mu m while keeping the field of view of 450 mu m x 450 mu m. The effectiveness of the method is demonstrated by imaging of fluorescent beads and aspergillus conidiophores. |
语种 | 英语 |
WOS记录号 | WOS:000908428100001 |
出版者 | FRONTIERS MEDIA SA |
源URL | [http://ir.opt.ac.cn/handle/181661/96302] ![]() |
专题 | 西安光学精密机械研究所_瞬态光学技术国家重点实验室 |
通讯作者 | Yu, Xianghua; Yao, Baoli |
作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian, Peoples R China |
推荐引用方式 GB/T 7714 | Gu, Shuangyu,Yu, Xianghua,Bai, Chen,et al. Multiple airy beams light-sheet fluorescence microscopy[J]. FRONTIERS IN PHYSICS,2022,10. |
APA | Gu, Shuangyu.,Yu, Xianghua.,Bai, Chen.,Min, Junwei.,Li, Runze.,...&Yao, Baoli.(2022).Multiple airy beams light-sheet fluorescence microscopy.FRONTIERS IN PHYSICS,10. |
MLA | Gu, Shuangyu,et al."Multiple airy beams light-sheet fluorescence microscopy".FRONTIERS IN PHYSICS 10(2022). |
入库方式: OAI收割
来源:西安光学精密机械研究所
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