中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Multiple airy beams light-sheet fluorescence microscopy

文献类型:期刊论文

作者Gu, Shuangyu1,2; Yu, Xianghua1,2; Bai, Chen2; Min, Junwei2; Li, Runze2; Yang, Yanlong2; Yao, Baoli1,2
刊名FRONTIERS IN PHYSICS
出版日期2022-12-23
卷号10
关键词light-sheet fluorescence microscopy volumetric imaging non-diffracting beam image contrast field of view
ISSN号2296-424X
DOI10.3389/fphy.2022.1111023
产权排序1
英文摘要

Light-sheet fluorescence microscopy (LSFM) is a kind of volumetric imaging methodology suited for long term living specimens at high temporal-spatial resolution. A single Airy beam (SAB) light-sheet can extend the field of view of Light-sheet fluorescence microscopy benefiting from its non-diffracting nature, but at the cost of out-of-focus background and low imaging contrast caused by side lobes illumination. Here, we propose a method to generate a sort of multiple Airy beams (MAB), which are linearly superimposed of multiple single Airy beams with different scale factors. Compared to the SAB light-sheet, the energy of the multiple Airy beams light-sheet is more concentrated on the focal plane of the detection objective, which can improve the imaging contrast and decrease the photodamage effect. Furthermore, we combined the complementary beam subtraction (CBS) strategy to increase the axial resolution, termed as multiple Airy beams-complementary beam subtraction method, which enables the axial resolution of 1.2 mu m while keeping the field of view of 450 mu m x 450 mu m. The effectiveness of the method is demonstrated by imaging of fluorescent beads and aspergillus conidiophores.

语种英语
WOS记录号WOS:000908428100001
出版者FRONTIERS MEDIA SA
源URL[http://ir.opt.ac.cn/handle/181661/96302]  
专题西安光学精密机械研究所_瞬态光学技术国家重点实验室
通讯作者Yu, Xianghua; Yao, Baoli
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China
2.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian, Peoples R China
推荐引用方式
GB/T 7714
Gu, Shuangyu,Yu, Xianghua,Bai, Chen,et al. Multiple airy beams light-sheet fluorescence microscopy[J]. FRONTIERS IN PHYSICS,2022,10.
APA Gu, Shuangyu.,Yu, Xianghua.,Bai, Chen.,Min, Junwei.,Li, Runze.,...&Yao, Baoli.(2022).Multiple airy beams light-sheet fluorescence microscopy.FRONTIERS IN PHYSICS,10.
MLA Gu, Shuangyu,et al."Multiple airy beams light-sheet fluorescence microscopy".FRONTIERS IN PHYSICS 10(2022).

入库方式: OAI收割

来源:西安光学精密机械研究所

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