A rapid and sensitive assay to quantify amino sugars, neutral sugars and uronic acid necromass biomarkers using pre-column derivatization, ultra-high-performance liquid chromatography and high-resolution mass spectrometry
文献类型：期刊论文
| 作者 | Salas, Erika1,2,4; Gorfer, Markus5; Bandian, Dragana5; Wang, Baorong3; Kaiser, Christina1; Wanek, Wolfgang1 |
| 刊名 | SOIL BIOLOGY & BIOCHEMISTRY
 |
| 出版日期 | 2023-02-01 |
| 卷号 | 177页码:9 |
| ISSN号 | 0038-0717 |
| 关键词 | Microbial necromass biomarkers Amino sugars Neutral sugars Uronic acids PMP derivatization UPLC-Orbitrap MS Isotopic enrichment precision |
| DOI | 10.1016/j.soilbio.2022.108927 |
| 通讯作者 | Salas, Erika(erika.kristel.salas.hernandez@univie.ac.at) |
| 英文摘要 | Microbial necromass comprises a large fraction of soil organic matter (SOM) due to the accumulation and stabilization of microbial residues from dead archaea, bacteria and fungi. Amino sugars, neutral sugars and uronic acids have been used as microbial necromass biomarkers to trace the origin and composition of microbial residues in the SOM pool. Due to the structural complexity of sugars, derivatization reactions and high-throughput analytical methods are required to separate and quantify these sugar-related compounds. Our aim was to develop a rapid and sensitive assay to measure amino sugar, neutral sugar and uronic acid compounds using pre-column 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatization. PMP-derivatives were separated and quantified via reversed phase (RP) ultra-high-performance liquid chromatography (UPLC) coupled to high-resolution Orbitrap mass spectrometry (MS). The method was validated and applied on hydrolyzed peptidoglycans and the biomass of archaeal, bacterial, fungal and plant species, as well as with soils. This developed PMP method allowed the separation and quantification of 18 sugar-related compounds, including four amino sugars, three N-acetyl amino sugars, eight neutral sugars, and three uronic acids within 20 min. This PMP method showed a precision for isotope enrichment detection of 0.03-0.05 atom % 13C for D-glucose and D-glucosamine. This is the first time talosaminuronic acid (deriving from archaeal pseudopeptidoglycan) was identified and quantified using PMP derivatization. The application of this novel PMP method on pure hydrolyzed biomass and soils, showed the successful chromatographic and mass spectrometric separation and quantification of amino sugar, neutral sugar and uronic acid compounds. A multivariate analysis using these sugar-related PMP derivatives showed a clustering of the species according to their respective taxonomic group (archaea, gram-positive bacteria, gramnegative bacteria, fungi and plants). The modified PMP method can be applied to identify and quantify soil microbial necromass biomarkers, as well as their contribution to SOM. The sensitive isotope tracer detection allows tracing isotopically labeled materials into necromass biomarkers in SOM pools. |
| WOS关键词 | BACTERIAL RESIDUES ; MICROBIAL BIOMASS ; ORGANIC-MATTER ; SOIL ; FUNGAL ; CARBON ; PATHWAYS ; GLUCOSE |
| WOS研究方向 | Agriculture |
| 语种 | 英语 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| WOS记录号 | WOS:000913010400001 |
| 源URL | [http://ir.igsnrr.ac.cn/handle/311030/189168]  |
| 专题 | 中国科学院地理科学与资源研究所 |
| 通讯作者 | Salas, Erika |
| 作者单位 | 1.Univ Vienna, Ctr Microbiol & Environm Syst Sci, Div Terr Ecosyst Res, Vienna, Austria 2.Univ Vienna, Doctoral Sch Microbiol & Environm Sci, Vienna, Austria 3.Chinese Acad Sci & Minist Water Resources, Inst Soil & Water Conservat, State Key Lab Soil Eros & Dryland Farming Loess Pl, Yangling, Peoples R China 4.Univ Vienna, Ctr Microbiol & Environm Syst Sci, Djerassipl 1, A-1030 Vienna, Austria 5.AIT Austrian Inst Technol GmbH, Bioresources, Tulln, Austria |
| 推荐引用方式 GB/T 7714 | Salas, Erika,Gorfer, Markus,Bandian, Dragana,et al. A rapid and sensitive assay to quantify amino sugars, neutral sugars and uronic acid necromass biomarkers using pre-column derivatization, ultra-high-performance liquid chromatography and high-resolution mass spectrometry[J]. SOIL BIOLOGY & BIOCHEMISTRY,2023,177:9. |
| APA | Salas, Erika,Gorfer, Markus,Bandian, Dragana,Wang, Baorong,Kaiser, Christina,&Wanek, Wolfgang.(2023).A rapid and sensitive assay to quantify amino sugars, neutral sugars and uronic acid necromass biomarkers using pre-column derivatization, ultra-high-performance liquid chromatography and high-resolution mass spectrometry.SOIL BIOLOGY & BIOCHEMISTRY,177,9. |
| MLA | Salas, Erika,et al."A rapid and sensitive assay to quantify amino sugars, neutral sugars and uronic acid necromass biomarkers using pre-column derivatization, ultra-high-performance liquid chromatography and high-resolution mass spectrometry".SOIL BIOLOGY & BIOCHEMISTRY 177(2023):9. |
入库方式： OAI收割
来源：地理科学与资源研究所
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