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Caragana rosea Turcz Methanol Extract Inhibits Lipopolysaccharide-Induced Inflammatory Responses by Suppressing the TLR4/NF-kappa B/IRF3 Signaling Pathways

文献类型：期刊论文

作者Mitra, Ankita; Ahuja, Akash; Rahmawati, Laily; Kim, Han Gyung; Woo, Byoung Young1; Hong, Yong Deog1; Hossain, Mohammad Amjad; Zhang, Zhiyun5; Kim, Soo-Yong; Lee, Jongsung
刊名MOLECULES
出版日期2021
卷号26期号:21
关键词Caragana rosea Turcz (Cr-ME) anti-inflammatory activity NF-kappa B and IRF3 signaling pathways
DOI10.3390/molecules26216660
文献子类Article
英文摘要Caragana rosea Turcz, which belongs to the Leguminosae family, is a small shrub found in Northern and Eastern China that is known to possess anti-inflammatory properties and is used to treat fever, asthma, and cough. However, the underlying molecular mechanisms of its anti-inflammatory effects are unknown. Therefore, we used lipopolysaccharide (LPS) in RAW264.7 macrophages to investigate the molecular mechanisms that underlie the anti-inflammatory activities of a methanol extract of Caragana rosea (Cr-ME). We showed that Cr-ME reduced the production of nitric oxide (NO) and mRNA levels of iNOS, TNF-alpha, and IL-6 in a concentration-dependent manner. We also found that Cr-ME blocked MyD88- and TBK1-induced NF-kappa B and IRF3 promoter activity, suggesting that it affects multiple targets. Moreover, Cr-ME reduced the phosphorylation levels of I kappa B alpha, IKK alpha/beta and IRF3 in a time-dependent manner and regulated the upstream NF-kappa B proteins Syk and Src, and the IRF3 protein TBK1. Upon overexpression of Src and TBK1, Cr-ME stimulation attenuated the phosphorylation of the NF-kappa B subunits p50 and p65 and IRF3 signaling. Together, our results suggest that the anti-inflammatory activity of Cr-ME occurs by inhibiting the NF-kappa B and IRF3 signaling pathways.
学科主题Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
电子版国际标准刊号1420-3049
出版地BASEL
WOS关键词TOLL-LIKE RECEPTORS ; KAPPA-B ; TYROSINE KINASE ; IN-VITRO ; RECOGNITION ; EXPRESSION ; CELLS ; ASSAY ; IRF3 ; L.
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
出版者MDPI
WOS记录号WOS:000719453400001
资助机构Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [2017R1A6A1A03015642] ; National Institute of Biological Resources ; Ministry of Environment [NIBR202006202] ; Republic of Korea
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/26670]  
专题系统与进化植物学国家重点实验室
作者单位1.Sungkyunkwan Univ, Dept Integrat Biotechnol, Suwon 16419, South Korea
2.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China
3.Korea Res Inst Biosci & Biotechnol, Int Biol Mat Res Ctr, Daejeon 34141, South Korea
4.AmorePacif R&D Ctr, Yongin 17074, South Korea
5.Chonbuk Natl Univ, Coll Med, Dept Vet Physiol, Iksan 54596, South Korea
推荐引用方式
GB/T 7714		 Mitra, Ankita,Ahuja, Akash,Rahmawati, Laily,et al. Caragana rosea Turcz Methanol Extract Inhibits Lipopolysaccharide-Induced Inflammatory Responses by Suppressing the TLR4/NF-kappa B/IRF3 Signaling Pathways[J]. MOLECULES,2021,26(21).
APA Mitra, Ankita.,Ahuja, Akash.,Rahmawati, Laily.,Kim, Han Gyung.,Woo, Byoung Young.,...&Cho, Jae Youl.(2021).Caragana rosea Turcz Methanol Extract Inhibits Lipopolysaccharide-Induced Inflammatory Responses by Suppressing the TLR4/NF-kappa B/IRF3 Signaling Pathways.MOLECULES,26(21).
MLA Mitra, Ankita,et al."Caragana rosea Turcz Methanol Extract Inhibits Lipopolysaccharide-Induced Inflammatory Responses by Suppressing the TLR4/NF-kappa B/IRF3 Signaling Pathways".MOLECULES 26.21(2021).

入库方式： OAI收割

来源：植物研究所

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