An Optimized Transformation System and Functional Test of CYC-Like TCP Gene CpCYC in Chirita pumila (Gesneriaceae)
文献类型：期刊论文
| 作者 | Liu, Jing; Wang, Juan-Juan; Wu, Jie; Wang, Yang; Liu, Qi; Liu, Fang-Pu; Yang, Xia; Wang, Yin-Zheng
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| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2021 |
| 卷号 | 22期号:9 |
| 关键词 | Chirita pumila CpCYC evo-devo functional test genetic transformation Gesneriaceae a new model |
| DOI | 10.3390/ijms22094544 |
| 文献子类 | Article |
| 英文摘要 | The development of an ideal model plant located at a key phylogenetic node is critically important to advance functional and regulatory studies of key regulatory genes in the evolutionary developmental (evo-devo) biology field. In this study, we selected Chirita pumila in the family Gesneriaceae, a basal group in Lamiales, as a model plant to optimize its genetic transformation system established previously by us through investigating a series of factors and further conduct functional test of the CYC-like floral symmetry gene CpCYC. By transforming a RNAi:CpCYC vector, we successfully achieved the desired phenotypes of upright actinomorphic flowers, which suggest that CpCYC actually determines the establishment of floral zygomorphy and the horizontal orientation of flowers in C. pumila. We also confirmed the activities of CpCYC promoter in dorsal petals, dorsal/lateral staminodes, as well as the pedicel by transferring a CpCYC promoter:GUS vector into C. pumila. Furthermore, we testified the availability of a transient gene expression system using C. pumila mesophyll protoplasts. The improved transformation system together with the inherent biological features would make C. pumila an attractive new model in functional and regulatory studies for a broad range of evo-devo issues. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| 出版地 | BASEL |
| 电子版国际标准刊号 | 1422-0067 |
| WOS关键词 | AGROBACTERIUM-MEDIATED TRANSFORMATION ; FLORAL ZYGOMORPHY ; EFFICIENT TRANSFORMATION ; NICOTIANA-BENTHAMIANA ; ANTIBIOTIC TIMENTIN ; PLANT-REGENERATION ; REGULATORY CHANGES ; SYMMETRY GENES ; PETAL SHAPE ; CLADE GENES |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000650369900001 |
| 出版者 | MDPI |
| 资助机构 | National Natural Science Foundation of China [31670217, 31900196, 31970239, 31530003] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26695]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Wang, Juan-Juan 2.[Liu, Jing 3.Liu, Fang-Pu 4.Liu, Qi 5.Wang, Yang 6.Wu, Jie 7.Univ Chinese Acad Sci, Beijing 101408, Peoples R China 8.[Wang, Juan-Juan 9.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 10.Yang, Xia |
| 推荐引用方式 GB/T 7714 | Liu, Jing,Wang, Juan-Juan,Wu, Jie,et al. An Optimized Transformation System and Functional Test of CYC-Like TCP Gene CpCYC in Chirita pumila (Gesneriaceae)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(9). |
| APA | Liu, Jing.,Wang, Juan-Juan.,Wu, Jie.,Wang, Yang.,Liu, Qi.,...&Wang, Yin-Zheng.(2021).An Optimized Transformation System and Functional Test of CYC-Like TCP Gene CpCYC in Chirita pumila (Gesneriaceae).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(9). |
| MLA | Liu, Jing,et al."An Optimized Transformation System and Functional Test of CYC-Like TCP Gene CpCYC in Chirita pumila (Gesneriaceae)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.9(2021). |
入库方式： OAI收割
来源：植物研究所
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