Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor
文献类型：期刊论文
| 作者 | Li, Donghai1; Pan, Cheng; Lu, Jianjun; Zaman, Wajid1,2; Zhao, Huayan3; Zhang, Jixing4; Lu, Shiyou3 |
| 刊名 | MOLECULES
 |
| 出版日期 | 2021 |
| 卷号 | 26期号:10 |
| 关键词 | castor lupeol transcriptome auxin terpene |
| DOI | 10.3390/molecules26102978 |
| 文献子类 | Article |
| 英文摘要 | Lupeol, a natural lupane-type pentacyclic triterpene, possesses various pharmacological properties, and its production attracts attention. Significant quantities of lupeol are deposited on the castor aerial organ surface and are easily extractable as a predominant wax constituent. Thus, castor might be considered as a potential bioreactor for the production of lupeol. The lupeol biosynthesis pathway is well known, but how it is regulated remains largely unknown. Among large numbers of castor cultivars, we targeted one accession line (337) with high levels of lupeol on its stem surface and low levels thereof on its hypocotyl surface, implicating that lupeol synthesis is differentially regulated in the two organs. To explore the underlying mechanisms, we did comparative transcriptome analysis of the first internode of 337 stem and the upper hypocotyl. Our results show that large amounts of auxin-related genes are differentially expressed in both parts, implying some possible interactions between auxin and lupeol production. We also found that several auxin-responsive cis-elements are present in promoter regions of HMGR and LUS genes encoding two key enzymes involved in lupeol production. Furthermore, auxin treatments apparently induced the expression levels of RcHMGR and RcLUS. Furthermore, we observed that auxin treatment significantly increased lupeol contents, whereas inhibiting auxin transport led to an opposite phenotype. Our study reveals some relationships between hormone activity and lupeol synthesis and might provide a promising way for improving lupeol yields in castor. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| 出版地 | BASEL |
| 电子版国际标准刊号 | 1420-3049 |
| WOS关键词 | GENES ; DERIVATIVES ; EXPRESSION ; HYPOCOTYL |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000655086400001 |
| 出版者 | MDPI |
| 资助机构 | Natural Science Foundation of China [32070282, 31760399] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26698]  |
| 专题 | 植物研究所_系统与进化植物学研究中心_系统与进化植物学研究中心_学位论文 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 4.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China 5.Inner Mongolia Univ Nationalities, Coll Life Sci & Food Engn, Tongliao 028000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Donghai,Pan, Cheng,Lu, Jianjun,et al. Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor[J]. MOLECULES,2021,26(10). |
| APA | Li, Donghai.,Pan, Cheng.,Lu, Jianjun.,Zaman, Wajid.,Zhao, Huayan.,...&Lu, Shiyou.(2021).Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor.MOLECULES,26(10). |
| MLA | Li, Donghai,et al."Lupeol Accumulation Correlates with Auxin in the Epidermis of Castor".MOLECULES 26.10(2021). |
入库方式： OAI收割
来源：植物研究所
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