Molecular Cloning and Functional Analysis of GmLACS2-3 Reveals Its Involvement in Cutin and Suberin Biosynthesis along with Abiotic Stress Tolerance
文献类型：期刊论文
| 作者 | Ayaz, Asma; Huang, Haodong; Zheng, Minglu; Zaman, Wajid4; Li, Donghai; Saqib, Saddam1,3; Zhao, Huayan; Lu, Shiyou |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2021 |
| 卷号 | 22期号:17 |
| 关键词 | long-chain acyl-CoA synthetases Glycine max abiotic stresses cutin cuticular wax suberin |
| DOI | 10.3390/ijms22179175 |
| 文献子类 | Article |
| 英文摘要 | Cutin and wax are the main precursors of the cuticle that covers the aerial parts of plants and provide protection against biotic and abiotic stresses. Long-chain acyl-CoA synthetases (LACSs) play diversified roles in the synthesis of cutin, wax, and triacylglycerol (TAG). Most of the information concerned with LACS functions is obtained from model plants, whereas the roles of LACS genes in Glycine max are less known. Here, we have identified 19 LACS genes in Glycine max, an important crop plant, and further focused our attention on 4 LACS2 genes (named as GmLACS2-1, 2, 3, 4, respectively). These GmLACS2 genes display different expression patterns in various organs and also show different responses to abiotic stresses, implying that these genes might play diversified functions during plant growth and against stresses. To further identify the role of GmLACS2-3, greatly induced by abiotic stresses, we transformed a construct containing its full length of coding sequence into Arabidopsis. The expression of GmLACS2-3 in an Arabidopsis atlacs2 mutant greatly suppressed its phenotype, suggesting it plays conserved roles with that of AtLACS2. The overexpression of GmLACS2-3 in wild-type plants significantly increased the amounts of cutin and suberin but had little effect on wax amounts, indicating the specific role of GmLACS2-3 in the synthesis of cutin and suberin. In addition, these GmLACS2-3 overexpressing plants showed enhanced drought tolerance. Taken together, our study deepens our understanding of the functions of LACS genes in different plants and also provides a clue for cultivating crops with strong drought resistance. |
| 学科主题 | Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary |
| 电子版国际标准刊号 | 1422-0067 |
| 出版地 | BASEL |
| WOS关键词 | ACYL-COENZYME ; WAX ; GENE ; IDENTIFICATION ; SYNTHETASE ; DEFICIENCY ; EXPRESSION |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| 出版者 | MDPI |
| WOS记录号 | WOS:000694265900001 |
| 资助机构 | Natural Science Foundation of China [32070282] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26770]  |
| 专题 | 系统与进化植物学国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Lushan Bot Garden, Nanchang 332900, Jiangxi, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 4.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ayaz, Asma,Huang, Haodong,Zheng, Minglu,et al. Molecular Cloning and Functional Analysis of GmLACS2-3 Reveals Its Involvement in Cutin and Suberin Biosynthesis along with Abiotic Stress Tolerance[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2021,22(17). |
| APA | Ayaz, Asma.,Huang, Haodong.,Zheng, Minglu.,Zaman, Wajid.,Li, Donghai.,...&Lu, Shiyou.(2021).Molecular Cloning and Functional Analysis of GmLACS2-3 Reveals Its Involvement in Cutin and Suberin Biosynthesis along with Abiotic Stress Tolerance.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,22(17). |
| MLA | Ayaz, Asma,et al."Molecular Cloning and Functional Analysis of GmLACS2-3 Reveals Its Involvement in Cutin and Suberin Biosynthesis along with Abiotic Stress Tolerance".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22.17(2021). |
入库方式： OAI收割
来源：植物研究所
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