Cytosine methylation of the FWA promoter promotes direct in vitro shoot regeneration in Arabidopsis thaliana
文献类型：期刊论文
| 作者 | Dai, Xuehuan; Wang, Jing; Song, Yuguang1; Liu, Zhenhua3; Xue, Tao5; Qiao, Meng; Yu, Yanchong6; Xin, Wei2; Xiang, Fengning |
| 刊名 | JOURNAL OF INTEGRATIVE PLANT BIOLOGY
 |
| 出版日期 | 2021 |
| 卷号 | 63期号:8页码:1491-1504 |
| 关键词 | DNA methylation epigenetic modification FLOWERING WAGENINGEN (FWA) shoot regeneration WOX9 |
| ISSN号 | 1672-9072 |
| DOI | 10.1111/jipb.13156 |
| 文献子类 | Article |
| 英文摘要 | Epigenetic modifications within promoter sequences can act as regulators of gene expression. Shoot regeneration is influenced by both DNA methylation and histone methylation, but the mechanistic basis of this regulation is obscure. Here, we identified 218 genes related to the regeneration capacity of callus that were differentially transcribed between regenerable calli (RC) and non-regenerable calli (NRC) in Arabidopsis thaliana. An analysis of the promoters of five of the differentially expressed genes (FWA, ACC1, TFL1, MAX3, and GRP3) pointed to an inverse relationship between cytosine methylation and transcription. The FWA promoter was demethylated and highly expressed in NRC, whereas it was methylated and expressed at low levels in RC. Explants of the hypomethylation mutants fwa-1 and fwa-2 showed strong levels of FWA expression and regenerated less readily than the wild type, suggesting that FWA inhibits direct in vitro shoot regeneration. WUSCHEL-RELATED HOMEOBOX 9 (WOX9), which is required for shoot apical meristem formation, was directly repressed by FWA. Overexpressing WOX9 partly rescued the shoot regeneration defect of fwa-2 plants. These findings suggest that cytosine methylation of the FWA promoter forms part of the regulatory system governing callus regenerability and direct in vitro shoot regeneration. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | HOBOKEN |
| 电子版国际标准刊号 | 1744-7909 |
| WOS关键词 | LATE-FLOWERING PHENOTYPE ; STEM-CELL FATE ; DNA METHYLATION ; GENE-EXPRESSION ; ORGANOGENESIS ; EMBRYOGENESIS ; COMPETENCE ; MUTANTS ; ALLELES ; WUSCHEL |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000686294800009 |
| 出版者 | WILEY |
| 资助机构 | National Natural Science Foundation of China [U1906203, 31770317, 31970189] ; National Transgenic Project of China [2016ZX08010002-009, 2018ZX08009-14B] ; China Postdoctoral Science Foundation [2019M662331] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26345]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Shandong Univ, Sch Life Sci, Key Lab Plant Dev & Environm Adaptat Biol, Minist Educ, Qingdao 266237, Peoples R China 2.Qingdao Agr Univ, Qingdao 266109, Peoples R China 3.Qufu Normal Univ, Qufu 273165, Shandong, Peoples R China 4.Chinese Acad Sci, Inst Bot, Beijing 100101, Peoples R China 5.Shandong Univ Tradit Chinese Med, Jinan 250355, Peoples R China 6.Huaibei Normal Univ, Huaibei 235000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dai, Xuehuan,Wang, Jing,Song, Yuguang,et al. Cytosine methylation of the FWA promoter promotes direct in vitro shoot regeneration in Arabidopsis thaliana[J]. JOURNAL OF INTEGRATIVE PLANT BIOLOGY,2021,63(8):1491-1504. |
| APA | Dai, Xuehuan.,Wang, Jing.,Song, Yuguang.,Liu, Zhenhua.,Xue, Tao.,...&Xiang, Fengning.(2021).Cytosine methylation of the FWA promoter promotes direct in vitro shoot regeneration in Arabidopsis thaliana.JOURNAL OF INTEGRATIVE PLANT BIOLOGY,63(8),1491-1504. |
| MLA | Dai, Xuehuan,et al."Cytosine methylation of the FWA promoter promotes direct in vitro shoot regeneration in Arabidopsis thaliana".JOURNAL OF INTEGRATIVE PLANT BIOLOGY 63.8(2021):1491-1504. |
入库方式： OAI收割
来源：植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。