中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 植物研究所
  3. 中国科学院植物研究所
  4. 中科院植物分子生理学重点实验室
The vernalization-induced long non-coding RNA VAS functions with the transcription factor TaRF2b to promote TaVRN1 expression for flowering in hexaploid wheat

文献类型：期刊论文

作者Xu, Shujuan2; Dong, Qi2; Deng, Min2,7; Lin, Dexing2,6; Xiao, Jun4,7; Cheng, Peilei5; Xing, Lijing; Niu, Yuda; Gao, Caixia6; Zhang, Wenhao2,8
刊名MOLECULAR PLANT
出版日期2021
卷号14期号:9页码:1525-1538
ISSN号1674-2052
关键词winter wheat vernalization flowering long non-coding RNA chromosome loop
DOI10.1016/j.molp.2021.05.026
文献子类Article
英文摘要Vernalization is a physiological process in which prolonged cold exposure establishes flowering competence in winter plants. In hexaploid wheat, TaVRN1 is a cold-induced key regulator that accelerates floral transition. However, the molecular mechanism underlying the gradual activation of TaVRN1 during the vernalization process remains unknown. In this study, we identified the novel transcript VAS (TaVRN1 alternative splicing) as a non-coding RNA derived from the sense strand of the TaVRN1 gene only in winter wheat, which regulates TaVRN1 transcription for flowering. VAS was induced during the early period of vernalization, and its overexpression promoted TaVRN1 expression to accelerate flowering in winter wheat. VAS physically associates with TaRF2b and facilitates docking of the TaRF2b-TaRF2a complex at the TaVRN1 promoter during the middle period of vernalization. TaRF2b recognizes the Sp1 motif within the TaVRN1 proximal promoter region, which is gradually exposed along with the disruption of a loop structure at the TaVRN1 locus during vernalization, to activate the transcription of TaVRN1. The tarf2b mutants exhibited delayed flowering, whereas transgenic wheat lines overexpressing TaRF2b showed earlier flowering. Taken together, our data reveal a distinct regulatory mechanism by which a long non-coding RNA facilitates the transcription factor targeting to regulate wheat flowering, providing novel insights into the vernalization process and a potential target for wheat genetic improvement.
学科主题Biochemistry & Molecular Biology ; Plant Sciences
电子版国际标准刊号1752-9867
出版地CAMBRIDGE
WOS关键词SPRING GROWTH HABIT ; ALLELIC VARIATION ; FLC ; RF2A ; GENE ; RESPONSES ; ARABIDOPSIS ; REPRESSOR ; PROTEIN ; COOLAIR
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
出版者CELL PRESS
WOS记录号WOS:000692963200018
资助机构NSFC [31788103] ; National Natural Science Foundation of China [31970529] ; China Postdoctoral Science Foundation [2019M650892]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/26348]  
专题中科院植物分子生理学重点实验室
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
3.Chinese Acad Sci, CAS JIC Ctr Excellence Plant & Microbial Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
4.Nanjing Agr Univ, Coll Hort, Key Lab Landscaping, Minist Agr, Nanjing 210095, Peoples R China
5.Chinese Acad Sci, Innovat Acad Seed Design, Ctr Genome Editing, Inst Genet & Dev Biol,State Key Lab Plant Cell &, Beijing 100101, Peoples R China
6.Chinese Acad Sci, Innovat Acad Seed Design, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China
7.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing 100093, Peoples R China
8.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Xu, Shujuan,Dong, Qi,Deng, Min,et al. The vernalization-induced long non-coding RNA VAS functions with the transcription factor TaRF2b to promote TaVRN1 expression for flowering in hexaploid wheat[J]. MOLECULAR PLANT,2021,14(9):1525-1538.
APA Xu, Shujuan.,Dong, Qi.,Deng, Min.,Lin, Dexing.,Xiao, Jun.,...&Chong, Kang.(2021).The vernalization-induced long non-coding RNA VAS functions with the transcription factor TaRF2b to promote TaVRN1 expression for flowering in hexaploid wheat.MOLECULAR PLANT,14(9),1525-1538.
MLA Xu, Shujuan,et al."The vernalization-induced long non-coding RNA VAS functions with the transcription factor TaRF2b to promote TaVRN1 expression for flowering in hexaploid wheat".MOLECULAR PLANT 14.9(2021):1525-1538.

入库方式： OAI收割

来源：植物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。