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The RING E3 ligase CLG1 targets GS3 for degradation via the endosome pathway to determine grain size in rice

文献类型：期刊论文

作者Yang, Wensi2; Wu, Kun3; Wang, Bo2; Liu, Huanhuan2; Guo, Siyi2; Guo, Xiaoyu2; Luo, Wei; Sun, Shengyuan1,7; Ouyang, Yidan1,4,7; Fu, Xiangdong3
刊名MOLECULAR PLANT
出版日期2021
卷号14期号:10页码:1699-1713
关键词grain size CLG1 GS3 E3 ligase endosome
ISSN号1674-2052
DOI10.1016/j.molp.2021.06.027
文献子类Article
英文摘要G-protein signaling and ubiquitin-dependent degradation are both involved in grain development in rice, but how these pathways are coordinated in regulating this process is unknown. Here, we show that Chang Li Geng 1 (CLG1), which encodes an E3 ligase, regulates grain size by targeting the G gamma protein GS3, a negative regulator of grain length, for degradation. Overexpression of CLG1 led to increased grain length, while overexpression of mutated CLG1with changes in three conserved amino acids decreased grain length. Wefound that CLG1 physically interacts with and ubiquitinats GS3which is subsequently degraded through the endosome degradation pathway, leading to increased grain size. Furthermore, we identified a critical SNP in the exon 3 of CLG1 that is significantly associated with grain size variation in a core collection of cultivated rice. This SNP results in an amino acid substitution from Arg to Ser at position 163 of CLG1 that enhances the E3 ligase activity of CLG1 and thus increases rice grain size. Both the expression level of CLG1 and the SNP CLG(1163S) may be useful variations for manipulating grain size in rice.
学科主题Biochemistry & Molecular Biology ; Plant Sciences
出版地CAMBRIDGE
电子版国际标准刊号1752-9867
WOS关键词PROTEIN INTERACTIONS ; NEGATIVE REGULATOR ; NATURAL VARIATION ; RARE ALLELE ; ORGAN SIZE ; MAJOR QTL ; UBIQUITIN ; YIELD ; SYSTEM ; TOLERANCE
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000704593500015
出版者CELL PRESS
资助机构National Key Research and Development Program [2016YFD0100901, 2016YFD0100903] ; Earmarked Fund for the China Agricultural Research System [CARS01-05]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/26364]  
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
2.Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Hubei Hongshan Lab, Wuhan 430070, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Huazhong Agr Univ, Natl Ctr Plant Gene Res Wuhan, Wuhan 430070, Peoples R China
5.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China
6.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China
7.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Wensi,Wu, Kun,Wang, Bo,et al. The RING E3 ligase CLG1 targets GS3 for degradation via the endosome pathway to determine grain size in rice[J]. MOLECULAR PLANT,2021,14(10):1699-1713.
APA Yang, Wensi.,Wu, Kun.,Wang, Bo.,Liu, Huanhuan.,Guo, Siyi.,...&Xu, Yunyuan.(2021).The RING E3 ligase CLG1 targets GS3 for degradation via the endosome pathway to determine grain size in rice.MOLECULAR PLANT,14(10),1699-1713.
MLA Yang, Wensi,et al."The RING E3 ligase CLG1 targets GS3 for degradation via the endosome pathway to determine grain size in rice".MOLECULAR PLANT 14.10(2021):1699-1713.

入库方式： OAI收割

来源：植物研究所

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