Proteomic Analysis of Desiccation Tolerance and Its Re-Establishment in Different Embryo Axis Tissues of Germinated Pea Seeds
文献类型：期刊论文
| 作者 | Wang, Wei-Qing; Wang, Yue; Song, Xian-Jun ; Zhang, Qi; Cheng, Hong-Yan; Liu, Jun; Song, Song-Quan
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| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2021 |
| 卷号 | 20期号:5页码:2352-2363 |
| 关键词 | dehydration desiccation tolerance embryo axis Pisum sativum proteomics seed germination |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/acs.jproteome.0c00860 |
| 文献子类 | Article |
| 英文摘要 | The model of loss and re-establishment of desiccation tolerance (DT) in germinated seeds has been well developed to explore the mechanisms associated with DT, but little attention has been paid to the tissue variation in this model. Herein, we investigated DT in different embryo axis tissues of germinated pea seeds and its re-establishment by poly(ethylene glycol) (PEG) treatment and then employed an iTRAQ-based proteomic method to explore the underlying mechanisms. DT varied among the four embryo axis parts of germinated seeds: epicotyl > hypocotyl-E (hypocotyl part attached to the epicotyl) > hypocotyl-R (hypocotyl part attached to the radicle) > radicle. Meanwhile, PEG treatment of germinated seeds resulted in a differential extent of DT re-establishment in these tissues. Proteins involved in detoxification and stress response were enriched in desiccation-tolerant hypocotyls-E and epicotyls of germinated seeds, respectively. Upon rehydration, proteome change during dehydration was recovered in the hypocotyls-E but not in the radicles. PEG treatment of germinated seeds led to numerous changes in proteins, in abundance in desiccation-sensitive radicles and hypocotyls-R, of which many accumulated in the hypocotyls-E and epicotyls before the treatment. We hypothesized that accumulation of groups 1 and 5 LEA proteins and proteins related to detoxification, ABA, ethylene, and calcium signaling contributed mainly to the variation of DT in different tissues and its re-establishment. |
| 学科主题 | Biochemical Research Methods |
| 出版地 | WASHINGTON |
| 电子版国际标准刊号 | 1535-3907 |
| WOS关键词 | EMBRYOGENESIS ABUNDANT PROTEINS ; MEDICAGO-TRUNCATULA SEEDS ; HEAT-STABLE PROTEOME ; GENE-EXPRESSION ; MAIZE EMBRYOS ; ABSCISIC-ACID ; LEA PROTEINS ; RECALCITRANT ; ARABIDOPSIS ; SATIVUM |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000649269600018 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | National Natural Sciences Foundation of China [31400292, 31770357] ; Youth Innovation Promotion Association CAS ; Science and Technology Planning Project of Guangdong Province [2020B121201008] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26403]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Prov Key Lab Crop Germplasm Resources P, Guangzhou 510640, Peoples R China 2.[Wang, Wei-Qing 3.Wang, Yue 4.Song, Xian-Jun 5.Cheng, Hong-Yan 6.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 7.[Zhang, Qi 8.Liu, Jun |
| 推荐引用方式 GB/T 7714 | Wang, Wei-Qing,Wang, Yue,Song, Xian-Jun,et al. Proteomic Analysis of Desiccation Tolerance and Its Re-Establishment in Different Embryo Axis Tissues of Germinated Pea Seeds[J]. JOURNAL OF PROTEOME RESEARCH,2021,20(5):2352-2363. |
| APA | Wang, Wei-Qing.,Wang, Yue.,Song, Xian-Jun.,Zhang, Qi.,Cheng, Hong-Yan.,...&Song, Song-Quan.(2021).Proteomic Analysis of Desiccation Tolerance and Its Re-Establishment in Different Embryo Axis Tissues of Germinated Pea Seeds.JOURNAL OF PROTEOME RESEARCH,20(5),2352-2363. |
| MLA | Wang, Wei-Qing,et al."Proteomic Analysis of Desiccation Tolerance and Its Re-Establishment in Different Embryo Axis Tissues of Germinated Pea Seeds".JOURNAL OF PROTEOME RESEARCH 20.5(2021):2352-2363. |
入库方式： OAI收割
来源：植物研究所
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