OsFes1C, a potential nucleotide exchange factor for OsBiP1, is involved in the ER and salt stress responses
文献类型：期刊论文
| 作者 | Qian, Dandan; Xiong, Shuo1; Li, Mei1; Tian, Lihong; Qu, Le Qing1 |
| 刊名 | PLANT PHYSIOLOGY
 |
| 出版日期 | 2021 |
| 卷号 | 187期号:1页码:396-408 |
| ISSN号 | 0032-0889 |
| DOI | 10.1093/plphys/kiab263 |
| 文献子类 | Article |
| 英文摘要 | The endoplasmic reticulum (ER) quality control system monitors protein homeostasis and relies on the activity of many molecular chaperones. Binding immunoglobulin protein (BiP) is a major ER luminal chaperone that is involved in most functions of the organelle. BiP activity is tightly regulated by nucleotide exchange factors (NEFs). However, information about NEFs in plants is limited. We obtained a Fes1-like protein (OsFes1C) through isobaric tags for relative and absolute quantitation-based proteomics analysis of ER-stressed rice (Oryza sativa) seeds. Unlike its homologs in yeast and mammals, which are located in the cytosol and respond to heat stress, OsFes1C is an ER membrane protein and responds to ER and salt stresses. OsFes1C interacts directly with OsBiP1 and the interaction is inhibited by ATP but promoted by ADP, suggesting that OsFes1C acts as a potential NEF of OsBiP1 in vivo. Overexpression or suppression of OsFes1C led to hypersensitivity to ER stress and affected the growth of rice. Furthermore, we established that OsFes1C directly interacts with a putative salt response protein and is involved in the salt response. Taken together, our study marks an important step toward elucidating the functional mechanisms of an identified ER stress response factor in rice. |
| 学科主题 | Plant Sciences |
| 出版地 | CARY |
| 电子版国际标准刊号 | 1532-2548 |
| WOS关键词 | PROTEIN-QUALITY CONTROL ; ENDOPLASMIC-RETICULUM ; PHOTOMORPHOGENIC DEVELOPMENT ; GENE-EXPRESSION ; FACTOR FES1 ; HSP70 ; RICE ; DEGRADATION ; ACCUMULATION ; STABILITY |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000696244400037 |
| 出版者 | OXFORD UNIV PRESS INC |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of Sciences [XDA24010400] ; National Program of Transgenic Variety Development of China [2016ZX08001-006] ; Natural Science Foundation of China [31801267] |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26447]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qian, Dandan,Xiong, Shuo,Li, Mei,et al. OsFes1C, a potential nucleotide exchange factor for OsBiP1, is involved in the ER and salt stress responses[J]. PLANT PHYSIOLOGY,2021,187(1):396-408. |
| APA | Qian, Dandan,Xiong, Shuo,Li, Mei,Tian, Lihong,&Qu, Le Qing.(2021).OsFes1C, a potential nucleotide exchange factor for OsBiP1, is involved in the ER and salt stress responses.PLANT PHYSIOLOGY,187(1),396-408. |
| MLA | Qian, Dandan,et al."OsFes1C, a potential nucleotide exchange factor for OsBiP1, is involved in the ER and salt stress responses".PLANT PHYSIOLOGY 187.1(2021):396-408. |
入库方式： OAI收割
来源：植物研究所
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