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DEAD-BOX RNA HELICASE 27 regulates microRNA biogenesis, zygote division, and stem cell homeostasis

文献类型：期刊论文

作者Hou, Xiu-Li; Chen, Wen-Qiang; Hou, Yifeng3,4; Gong, Hua-Qin; Sun, Jing3,4; Wang, Zhen3,4; Zhao, Heng; Cao, Xiaofeng3,4; Song, Xiu-Fen; Liu, Chun-Ming
刊名PLANT CELL
出版日期2021
卷号33期号:1页码:66-84
ISSN号1040-4651
DOI10.1093/plcell/koaa001
文献子类Article
英文摘要After double fertilization, zygotic embryogenesis initiates a new life cycle, and stem cell homeostasis in the shoot apical meristem (SAM) and root apical meristem (RAM) allows plants to produce new tissues and organs continuously. Here, we report that mutations in DEAD-BOX RNA HELICASE 27 (RH27) affect zygote division and stem cell homeostasis in Arabidopsis (Arabidopsis thaliana). The strong mutant allele rh27-1 caused a zygote-lethal phenotype, while the weak mutant allele rh27-2 led to minor defects in embryogenesis and severely compromised stem cell homeostasis in the SAM and RAM. RH27 is expressed in embryos from the zygote stage, and in both the SAM and RAM, and RH27 is a nucleuslocalized protein. The expression levels of genes related to stem cell homeostasis were elevated in rh27-2 plants, alongside down-regulation of their regulatory microRNAs (miRNAs). Further analyses of rh27-2 plants revealed reduced levels of a large subset of miRNAs and their pri-miRNAs in shoot apices and root tips. In addition, biochemical studies showed that RH27 associates with pri-miRNAs and interacts with miRNA-biogenesis components, including DAWDLE, HYPONASTIC LEAVES 1, and SERRATE. Therefore, we propose that RH27 is a component of the microprocessor complex and is critical for zygote division and stem cell homeostasis.
学科主题Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
电子版国际标准刊号1532-298X
出版地CARY
WOS关键词ARABIDOPSIS-THALIANA ; PROTEIN INTERACTIONS ; FEEDBACK LOOP ; PRI-MIRNA ; SHOOT ; GENE ; EXPRESSION ; MERISTEM ; ENCODES ; CLV3
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
出版者OXFORD UNIV PRESS INC
WOS记录号WOS:000637288800009
资助机构National Transgenic Science and Technology Program [2019ZX08010-003] ; National Natural Science Foundation of China [31871455, 30625016, 31788103] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/26486]  
专题中科院植物分子生理学重点实验室
作者单位1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing 100081, Peoples R China
2.Chinese Acad Sci, Natl Ctr Plant Gene Res, CAS Ctr Excellence Mol Plant Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China
3.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China
4.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China
5.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China
推荐引用方式
GB/T 7714		 Hou, Xiu-Li,Chen, Wen-Qiang,Hou, Yifeng,et al. DEAD-BOX RNA HELICASE 27 regulates microRNA biogenesis, zygote division, and stem cell homeostasis[J]. PLANT CELL,2021,33(1):66-84.
APA Hou, Xiu-Li.,Chen, Wen-Qiang.,Hou, Yifeng.,Gong, Hua-Qin.,Sun, Jing.,...&Liu, Chun-Ming.(2021).DEAD-BOX RNA HELICASE 27 regulates microRNA biogenesis, zygote division, and stem cell homeostasis.PLANT CELL,33(1),66-84.
MLA Hou, Xiu-Li,et al."DEAD-BOX RNA HELICASE 27 regulates microRNA biogenesis, zygote division, and stem cell homeostasis".PLANT CELL 33.1(2021):66-84.

入库方式： OAI收割

来源：植物研究所

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