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Ectopic expression of the Arabidopsis florigen gene FLOWERING LOCUS T in seeds enhances seed dormancy via the GA and DOG1 pathways

文献类型：期刊论文

作者Chen, Fengying2; Li, Yu2; Li, Xiaoying; Li, Wenlong3; Xu, Jimei2; Cao, Hong; Wang, Zhi4; Li, Yong5; Soppe, Wim J. J.; Liu, Yongxiu
刊名PLANT JOURNAL
出版日期2021
卷号107期号:3页码:909-924
关键词seed dormancy FLOWERING LOCUS T TERMINAL FLOWER1 seed-specific expression gibberellin pathway DOG1 pathway
ISSN号0960-7412
DOI10.1111/tpj.15354
文献子类Article
英文摘要Ectopic expression of specific genes in seeds could be a tool for molecular design of crops to alter seed dormancy and germination, thereby improving production. Here, a seed-specific vector, 12S-pLEELA, was applied to study the roles of genes in Arabidopsis seeds. Transgenic lines containing FLOWERING LOCUS T (FT) driven by the 12S promoter exhibited significantly increased seed dormancy and earlier flowering. Mutated FT(Y85H) and TERMINAL FLOWER1 (TFL1) transgenic lines also showed increased seed dormancy but without altered flowering time. FT(Y85H) and TFL1 caused weaker seed dormancy enhancement compared to FT. The FT and TFL1 transgenic lines showed hypersensitivity to paclobutrazol, but not to abscisic acid in seed germination. The levels of bioactive gibberellin 3 (GA(3)) and GA(4) were significantly reduced, consistent with decreased expression of COPALYL DIPHOSPHATE SYNTHASE (CPS), KAURENE OXIDASE (KO), GIBBERELLIN 3-OXIDASE2 (GA3ox2), and GA20ox1 in p12S::FT lines. Exogenous GA(4+7) could recover the germination ability of FT transgenic lines. These results revealed that FT regulates GA biosynthesis. A genetic analysis indicated that the GA signaling regulator SPINDLY (SPY) is epistatic to FT in GA-mediated seed germination. Furthermore, DELAY OF GERMINATION1 (DOG1) showed significantly higher transcript levels in p12S::FT lines. Seed dormancy analysis of dog1-2 spy-3 p12S::FT-2 indicated that the combination of SPY and DOG1 is epistatic to FT in the regulation of dormancy. Overall, we showed that ectopic expression of FT and TFL1 in seeds enhances dormancy through affecting GA and DOG1 pathways.
学科主题Plant Sciences
出版地HOBOKEN
电子版国际标准刊号1365-313X
WOS关键词ABSCISIC-ACID ; NEGATIVE REGULATOR ; GIBBERELLIN BIOSYNTHESIS ; NATURAL VARIATION ; GERMINATION ; PROTEIN ; ABA ; TEMPERATURE ; INDUCTION ; TIME
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000667128300001
出版者WILEY
资助机构National Natural Science Foundation of China [31801090, 31571257]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/26515]  
专题中科院植物分子生理学重点实验室
作者单位1.Soppe, Wim J. J.] Rijk Zwaan Breeding BV, De Lier, Netherlands
2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China
3.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China
4.Sci & Technol Daily, Beijing, Peoples R China
5.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang, Peoples R China
6.Univ Freiburg, Inst Genet Epidemiol, Med Ctr, Freiburg, Germany
推荐引用方式
GB/T 7714		 Chen, Fengying,Li, Yu,Li, Xiaoying,et al. Ectopic expression of the Arabidopsis florigen gene FLOWERING LOCUS T in seeds enhances seed dormancy via the GA and DOG1 pathways[J]. PLANT JOURNAL,2021,107(3):909-924.
APA Chen, Fengying.,Li, Yu.,Li, Xiaoying.,Li, Wenlong.,Xu, Jimei.,...&Liu, Yongxiu.(2021).Ectopic expression of the Arabidopsis florigen gene FLOWERING LOCUS T in seeds enhances seed dormancy via the GA and DOG1 pathways.PLANT JOURNAL,107(3),909-924.
MLA Chen, Fengying,et al."Ectopic expression of the Arabidopsis florigen gene FLOWERING LOCUS T in seeds enhances seed dormancy via the GA and DOG1 pathways".PLANT JOURNAL 107.3(2021):909-924.

入库方式： OAI收割

来源：植物研究所

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