The rice VCS1 is identified as a molecula tool to mark and visualize the vegetative cell of pollen
文献类型：期刊论文
| 作者 | Wang, Yanli2; Wang, Tai1,2 ; Liu, Lingtong
|
| 刊名 | PLANT SIGNALING & BEHAVIOR
 |
| 出版日期 | 2021 |
| 卷号 | 16期号:8 |
| 关键词 | Pollen rice vegetative nucleus DUF3049 FANTASTIC FOUR |
| ISSN号 | 1559-2316 |
| DOI | 10.1080/15592324.2021.1924502 |
| 文献子类 | Article |
| 英文摘要 | Cell-type-specific markers are valuable tools to reveal developmental processes underlying cell differentiation during plant reproduction. Here we report the pollen vegetative cell marker gene VCS1 (Vegetative Cell Specific 1) of rice (Oryza sativa japonica). VCS1 was expressed specifically in late pollen and was predicted to encode a small FAF domain-containing protein of 205 amino acid residues (aa). The expression of reporter fusion proteins showed that VCS1 was exclusively targeted to the vegetative nucleus of pollen. Upon pollen germination, VCS1 lost vegetative nucleus localization, and appeared diffused in the vegetative cytoplasm of pollen grain but not in the pollen tube. T-DNA insertional mutation which disrupted the carboxyl-terminal 21 aa of VCS1 did not affect plant vegetative growth and pollen development, while destruction of VCS1 by CRISPR/Cas9 only moderately affect pollen viability. VCS1 is evolutionally conserved in monocots but appeared absent in dicotyledons. This study reveals a molecular tool for visualizing the vegetative cell of rice and possible other monocots, which has potential values in the genetic engineering of male-sterile lines. |
| 学科主题 | Biochemistry & Molecular Biology ; Plant Sciences |
| 出版地 | PHILADELPHIA |
| 电子版国际标准刊号 | 1559-2324 |
| WOS关键词 | DNA INSERTIONAL MUTAGENESIS ; GENE-EXPRESSION ; SPERM CELLS ; ZEA-MAYS ; PROMOTERS ; VECTORS ; PROTEIN ; SYSTEM |
| WOS研究方向 | Science Citation Index Expanded (SCI-EXPANDED) |
| 语种 | 英语 |
| WOS记录号 | WOS:000650561000001 |
| 出版者 | TAYLOR & FRANCIS INC |
| 资助机构 | National Natural Science Foundation of China [32000247] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences |
| 源URL | [http://ir.ibcas.ac.cn/handle/2S10CLM1/26581]  |
| 专题 | 中科院植物分子生理学重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yanli,Wang, Tai,Liu, Lingtong. The rice VCS1 is identified as a molecula tool to mark and visualize the vegetative cell of pollen[J]. PLANT SIGNALING & BEHAVIOR,2021,16(8). |
| APA | Wang, Yanli,Wang, Tai,&Liu, Lingtong.(2021).The rice VCS1 is identified as a molecula tool to mark and visualize the vegetative cell of pollen.PLANT SIGNALING & BEHAVIOR,16(8). |
| MLA | Wang, Yanli,et al."The rice VCS1 is identified as a molecula tool to mark and visualize the vegetative cell of pollen".PLANT SIGNALING & BEHAVIOR 16.8(2021). |
入库方式： OAI收割
来源：植物研究所
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